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Dr. Takuya Nishigaki
Investigador Titular B Tiempo Completo
Desarrollo de biosensores
Fisiología molecular del espermatozoide
Número de registros: 12
- Activos
- Vencidos
- Estudio de interacciones moleculares específicas usando proteínas fluorescentes, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2022 - 2024
- Caracterización funcional del intercambiador na+/h+ específico del espermatozoide, CONSEJO NACIONAL DE CIENCIA Y TECNOLOGÍA, 2019 - 2022
- Mecanismo de regulación del ph citosólico del espermatozoide mediante el intercambiador na+/h+ y un canal de protón voltaje dependiente., DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2019 - 2021
- Acoplamiento funcional entre intercambiador na+/h+específico al espermatozoide (snhe) y canal de ca2+ específico alespermatozaoide (catsper) en mamíferos, UNIVERSIDAD DE CALIFORNIA-MÉXICO, 2016 - 2018
- Estudio de tres proteínas específicas del espermatozoide, catsper, snhe y sac en la regulación del batido flagelar, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2016 - 2019
- Estudio de la regulación de la motilidad del espermatozoide por medio de herramientas ópticas, PRESUPUESTO UNAM, 2014 - 2020
- Mecanismo de regulación de la movilidad de los espermatozoides de mamíferos mediante ca2+, ph y amp cíclico, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2013 - 2016
- Estudio de la regulación de la movilidad del espermatozoide por ca2+, ph y amp cíclico usando herramientas ópticas, CONSEJO NACIONAL DE CIENCIA Y TECNOLOGÍA, 2012 - 2016
- Efectos de progesterona y prostaglandina e1 en la movilidad del espermatozoide de mamífero, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2010 - 2012
- Desarrollo de un sensor de fluorescencia para amp ciclico, CONSEJO NACIONAL DE CIENCIA Y TECNOLOGÍA, 2009
- Determinación de los cambios de ampc en el espermatozoide en tiempo real usando un sensor fluorescente, CONSEJO NACIONAL DE CIENCIA Y TECNOLOGÍA, 2007 - 2010
- Determinación cinética de los cambios de ampc en el espermatozoide usando un sensor fluorescente, DIRECCIÓN GENERAL DE ASUNTOS DEL PERSONAL ACADÉMICO/UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO, 2007 - 2009
Nivel C del PRIDE
Nivel III del SNII
Nivel III del SNII
Doctorado, en Biociencias, Instituto Tecnologico de Tokyo (1997)
Maestría, en Biociencias, Instituto Tecnologico de Tokyo (1994)
Licenciatura, Ciencias, Instituto Tecnologico de Tokyo (1992)
- Activos
- Graduados
-
Doctorado
-
Estudio de la actividad del intercambiador Na+ /H+ específico del espermatozoide en mamíferos.
-
Estudio estructural del dominio sensor de voltaje del intercambiador Na+/H+ especifico de espermatozoide de mamífero
-
Licenciatura
1 - Sanchez Guevara, Yoloxochitl (2024). Determinación semicuantitativa de niveles de calcio mitocondrial en espermatozoides de ratón usando indicadores fluorescentes .
Instituto de Biotecnologia,
Doctorado en Ciencias Biomédicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
2 - Hernandez Garduno, Sandra (2023). Estudio de la actividad del intercambiador Na+/H+ especifico del espermatozoide en mamiferos.
Instituto de Biotecnologia,
Doctorado en Ciencias Biomedicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
3 - Arcos Hernandez, Cesar (2023). Estudio funcional del dominio sensor de voltaje del intercambiador Na+/H+ especifico de espermatozoide de mamifero.
Instituto de Biotecnologia,
Doctorado en Bioquimicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
4 - López Velarde, Columba Alejandra (2020). Ensayo de unión tipo FRET in vivo entre un dominio de unión a nucleótidos cíclicos y AMPc .
Facultad de Ciencias,
Licenciatura en Biología,
UNAM.
*
Asesor: Nishigaki Shimizu, Takuya
5 - Romero Corpus, Francisco (2018). Estudio bioquimico y filogenetico del intercambiador Na+/H+ especifico del espermatozoide .
Instituto de Biotecnologia,
Doctorado en Bioquimicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
6 - Santana Calvo, Maria del Carmen (2016). Optimizacion del ensayo de union tipo FRET entre un dominio de union a nucleotidos ciclicos y AMPc .
Instituto de Biotecnologia,
Maestria en Ciencias Bioquimicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
7 - Monroy Lara, Juan Esteban (2016). Caracterizacion espectroscopica de proteinas quimericas construidas a partir de una proteina fluorescente y de un dominio de union a nucleotidos ciclocos : un intento para desarrollar un nuevo sensor fluorescente para el AMPC.
Instituto de Biotecnologia,
Maestria en Ciencias Bioquimicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
8 - Santana Calvo, Maria del Carmen (2015). Desarrollo de un ensayo de union tipo fret entre un dominio de union a nucleotidos ciclicos y AMPc.
Facultad de Ciencias,
Licenciatura en Biologia,
UNAM.
*
Asesor: Nishigaki Shimizu, Takuya
9 - Romero Corpus, Francisco (2012). Caracterizacion bioquimica del dominio de union a nucleotidos ciclicos del intercambiador na+/h+ especifico para espermatozoide de mamifero.
Instituto de Biotecnologia,
Maestria en Ciencias Bioquimicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
10 - Servin Vences, Martha Rocio (2011). Cambios en la concentracion del Ca2+ intracelular y en la movilidad del espermatozoide de humano inducidos por progesterona en fluidos de alta viscosidad.
Instituto de Biotecnologia,
Maestria en Ciencias Bioquimicas,
UNAM.
Asesor: Nishigaki Shimizu, Takuya
11 - Hernandez Rueda, Yadira Libertad (2011). Participacion del canal CatSper en el incremento de calcio intracelular inducido por PGE1 y progesterona en espermatozoides humanos .
Facultad de Ciencias,
Maestria en Ciencias Biologicas,
UNAM.
*
Asesor: Nishigaki Shimizu, Takuya
12 - Servin Vences, Martha Rocio (2009). Evaluacion de tres analogos fotoactivables de progesterona en la respuesta de la Ca2+ i en espermatozoide de humano .
Facultad de Ciencias,
Licenciatura en Biología,
UNAM.
*
Asesor: Nishigaki Shimizu, Takuya
Total de publicaciones: 60
- Tipo
- Fecha
Revisión
13 - Chavez,J.C., Carrasquel-Martinez,G., Hernandez-Garduno,S., Matamoros-Volante,A., Trevino,C.L., Nishigaki,T., Darszon,A. (2024). Cytosolic and Acrosomal pH Regulation in Mammalian Sperm.
Cells,
13 (10),
865.
Artículo
14 - Pacheco-Castillo,H., Zagal-Huerta,E.E., Acevedo-Fernandez,J.J., Negrete-Leon,E., Nishigaki,T., Beltran,C. (2024). Hyperglycemia adversely affects critical physiological events related to rat sperm capacitation.
Biochemical and Biophysical Research Communications,
734,
150610.
Artículo
15 - Sanchez-Guevara,Y., Oliver,E.I., Nishigaki,T. (2024). Ca2+ concentrations in mouse sperm mitochondria fluctuate according to the cytosol.
Reproduction,
167 (2),
e230237.
Artículo
16 - Arcos-Hernandez,C., Suarez-Delgado,E., Islas,L.D., Romero,F., Lopez-Gonzalez,I., Ai,H.W., Nishigaki,T. (2023). How to study a highly toxic protein to bacteria: A case of voltage sensor domain of mouse sperm-specific sodium/proton exchanger.
Protein Expression and Purification,
201,
106172.
Revisión
17 - Arcos-Hernandez,C., Nishigaki,T. (2023). Ion currents through the voltage sensor domain of distinct families of proteins.
Journal of Biological Physics,
49,
393-413.
Artículo
18 - Hernandez-Garduno,S., Chavez,J.C., Matamoros-Volante,A., Sanchez-Guevara,Y., Torres,P., Trevino,C.L., Nishigaki,T. (2022). Hyperpolarization induces cytosolic alkalization of mouse sperm flagellum probably through sperm Na+/H+ exchanger.
Reproduction,
164 (4),
125-134.
Artículo
19 - Chavez,J.C., Darszon,A., Trevino,C.L., Nishigaki,T. (2020). Quantitative intracellular pH determinations in single live mammalian spermatozoa using the ratiometric dye SNARF-5F.
Frontiers in Cell and Developmental Biology,
7,
366.
Revisión
20 - Darszon,A., Nishigaki,T., Lopez-Gonzalez,I., Visconti,P.E., Trevino,C.L. (2020). Differences and Similarities: The Richness of Comparative Sperm Physiology.
Physiology (Bethesda.),
35 (3),
196-208.
Artículo
21 - De La Rosa,J.A.M., Garcia-Castaneda,M., Nishigaki,T., Gomora,J.C., Mancilla-Percino,T., Avila,G. (2020). Interaction of MDIMP with the voltage-gated calcium channels.
Molecular Pharmacology,
98 (3),
211-221.
Artículo
22 - Garza-Lopez,E., Aldana,A., Darszon,A., Nishigaki,T., Lopez-Gonzalez,I. (2020). CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in its domains I/II.
Cell Calcium,
89,
102214.
Artículo
23 - Priego-Espinosa,D.A., Darszon,A., Guerrero,A., Gonzalez-Cota,A.L., Nishigaki,T., Martinez-Mekler,G., Carneiro,J. (2020). Modular analysis of the control of flagellar Ca2+-spike trains produced by CatSper and CaV channels in sea urchin sperm.
PLoS Computational Biology,
16 (3),
e1007605.
Artículo
24 - Kinoshita-Terauchi, N., Shiba, K., Terauchi, M., Romero, F., Ramirez-Gomez, H. V., Yoshida, M., Motomura, T., Kawai, H., Nishigaki, T. (2019). High potassium seawater inhibits ascidian sperm chemotaxis, but does not affect the male gamete chemotaxis of a brown alga.
Zygote,
27 (4),
225-231.
Artículo
Artículo
26 - Chavez, J.C., De la Vega-Beltran JL, Jose, O., Torres, P., Nishigaki, T., Trevino, C.L., Darszon, A. (2018). Acrosomal alkalization triggers Ca2+ release and acrosome reaction in mammalian spermatozoa.
Journal of Cellular Physiology,
233 (6),
4735-4747.
Artículo
27 - Santana-Calvo, C., Romero, F., Lopez-Gonzalez, I., Nishigaki, T. (2018). Robust evaluation of intermolecular FRET using a large Stokes shift fluorophore as a donor.
Biotechniques,
65 (4),
211-218.
Artículo
28 - Romero, F., Santana-Calvo, C., Sanchez-Guevara, Y., Nishigaki, T. (2017). FRET-based binding assay between a fluorescent cAMP analogue and a cyclic nucleotide-binding domain tagged with a CFP.
FEBS Letters,
591 (18),
2869-2878.
Artículo
29 - Arcos-Hernandez,C., Romero,F., Sanchez-Guevara,Y., Beltran,C., Nishigaki,T. (2016). FRET analysis using sperm-activating peptides tagged with fluorescent proteins reveals that ligand-binding sites exist as clusters.
Journal of Experimental Biology,
219 (Pt 4),
508-515.
Artículo
30 - Garza-Lopez,E., Chavez,J.C., Santana-Calvo,C., Lopez-Gonzalez,I., Nishigaki,T. (2016). Cd2+ sensitivity and permeability of a low voltage-activated Ca2+ channel with CatSper-like selectivity filter.
Cell Calcium,
60 (1),
41-50.
Artículo
31 - Gijsbers,A., Nishigaki,T., Sanchez-Puig,N. (2016). Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions.
Journal of Visualized Experiments JoVE,
116.
Artículo
32 - Nishigaki,T., Romero,F., Sanchez-Guevara,Y. (2016). CatSper, el canal de Ca2+ que regula el batido flagelar del espermatozoide en eucariontes.
Revista Iberoamericana de Ciencias,
3 (3),
28-36.
Artículo
33 - Garza-Lopez,E., Sanchez-Carranza,O., Nishigaki,T., Lopez-Gonzalez,I. (2015). Pharmacological identification of endogenous slo1 channel-á1 subunit complexes in CHO cells using three àKTx1 subfamily toxins.
International Journal of Pharmacy & Therapeutics,
6 (1),
11-24.
Revisión
34 - Beltran,C., Rodriguez-Miranda,E., Granados-Gonzalez,G., de De la Torre LG, Nishigaki,T., Darszon,A. (2014). Zn induces hyperpolarization by activation of a K channel and increases intracellular Ca and pH in sea urchin spermatozoa.
Developmental Biology,
394 (1),
15-23.
Revisión
35 - Nishigaki,T., Jose,O., Gonzalez-Cota,A.L., Romero,F., Trevino,C.L., Darszon,A. (2014). Intracellular pH in Sperm Physiology.
Biochemical and Biophysical Research Communications,
450 (3),
1149-1158.
Artículo
36 - Sanchez-Tusie,A.A., Vasudevan,S.R., Churchill,G.C., Nishigaki,T., Trevino,C.L. (2014). Characterization of NAADP-Mediated Calcium Signaling in Human Spermatozoa.
Biochemical and Biophysical Research Communications,
443 (2),
531-536.
Artículo
37 - Servin-Vences,M.R., Tatsu,Y., Ando,H., Guerrero,A., Yumoto,N., Darszon,A., Nishigaki,T. (2012). A caged progesterone analog alters intracellular Ca2+ and flagellar bending in human sperm.
Reproduction,
144 (1),
101-109.
Artículo
38 - Chavez,J.C., de Blas,G.A., de la Vega-Beltran,J.L., Nishigaki,T., Chirinos,M., Gonzalez-Gonzalez,M.E., Larrea,F., Solis,A., Darszon,A., Trevino,C.L. (2011). The opening of maitotoxin-sensitive calcium channels induces the acrosome reaction in human spermatozoa: differences from the zona pellucida.
Asian Journal of Andrology,
13,
159-165.
Revisión
39 - Darszon,A., Nishigaki,T., Beltran,C., Trevino,C.L. (2011). Calcium Channels in the Development, Maturation, and Function of Spermatozoa.
Physiological Reviews,
91 (4),
1305-1355.
Artículo
40 - Guerrero,A., Wood,C.D., Nishigaki,T., Carneiro,J., Darszon,A. (2010). Tuning sperm chemotaxis.
Biochemical Society Transactions,
38 (5),
1270-1274.
Artículo
41 - Guerrero,A., Nishigaki,T., Carneiro,J., Tatsu,Y., Wood,C.D., Darszon,A. (2010). Tuning sperm chemotaxis by calcium burst timing.
Developmental Biology,
344 (1),
52-65.
Artículo
42 - Darszon,A., Guerrero,A., Galindo,B.E., Nishigaki,T., Wood,C.D. (2008). Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility.
International Journal of Developmental Biology,
52 (5-6),
595-606.
Artículo
43 - de la Sancha,C.U., Martinez-Cadena,G., Lopez-Godinez,J., Castellano,L.E., Nishigaki,T., Darszon,A., Garcia-Soto,J. (2007). Rho-kinase (ROCK) in sea urchin sperm: Its role in regulating the intracellular pH during the acrosome reaction.
Biochemical and Biophysical Research Communications,
364 (3),
470-475 [Corrigendum en Volume 366, Issue 2, 2008, Page 604].
Artículo
44 - Rangel-Mata,F., Mendez-Marquez,R., Martinez-Cadena,G., Lopez-Godinez,J., Nishigaki,T., Darszon,A., Garcia-Soto,J. (2007). Rho, Rho-kinase, and the actin cytoskeleton regulate the Na(+)-H(+) exchanger in sea urchin eggs.
Biochemical and Biophysical Research Communications,
352 (1),
264-269.
Artículo
45 - Wood,C.D., Nishigaki,T., Tatsu,Y., Yumoto,N., Baba,S.A., Whitaker,M., Darszon,A. (2007). Altering the speract-induced ion permeability changes that generate flagellar Ca(2+) spikes regulates their kinetics and sea urchin sperm motility.
Developmental Biology,
306 (2),
525-537.
Revisión
46 - Darszon,A., Acevedo,J.J., Galindo,B.E., Hernandez-Gonzalez,E.O., Nishigaki,T., Trevino,C.L., Wood,C., Beltran,C. (2006). Sperm channel diversity and functional multiplicity.
Reproduction,
131 (6),
977-988.
Artículo
47 - Nishigaki,T., Wood,C.D., Shiba,K., Baba,S.A., Darszon,A. (2006). Stroboscopic illumination using light-emitting diodes reduces phototoxicity in fluorescence cell imaging.
Biotechniques,
41 (2),
191-197.
Artículo
48 - Trevino,C.L., de la Vega-Beltran,J.L., Nishigaki,T., Felix,R., Darszon,A. (2006). Maitotoxin potently promotes Ca(2+) influx in mouse spermatogenic cells and sperm, and induces the acrosome reaction.
Journal of Cellular Physiology,
206 (2),
449-456.
Revisión
49 - Darszon,A., Nishigaki,T., Wood,C., Trevino,C.L., Felix,R., Beltran,C. (2005). Calcium channels and ca(2+) fluctuations in sperm physiology.
International Review of Cytology,
243,
79-172.
Artículo
50 - Shiba,K., Ohmuro,J., Mogami,Y., Nishigaki,T., Wood,C.D., Darszon,A., Tatsu,Y., Yumoto,N., Baba,S.A. (2005). Sperm-activating Peptide induces asymmetric flagellar bending in sea urchin sperm.
Zoological Science,
22 (3),
293-299.
Artículo
51 - Wood,C.D., Nisihigaki,T., Furuta,T., Baba,S.A., Darszon,A. (2005). Real-time analysis of the role of Ca2+ in flagellar movement and motility in single sea urchin sperm.
Journal of Cell Biology,
169 (5),
725-731 .
Revisión
52 - Darszon,A., Wood,C.D., Beltran,C., Sanchez,D., Rodriguez,E., Gorelik,J., Korchev,Y.E., Nishigaki,T. (2004). Measuring ion fluxes in sperm.
Methods in Cell Biology,
74,
545-576.
Artículo
53 - Nishigaki,T., Wood,C.D., Tatsu,Y., Yumoto,N., Furuta,T., Elias,D., Shiba,K., Baba,S.A., Darszon,A. (2004). A sea urchin egg jelly peptide induces a cGMP-mediated decrease in sperm intracellular Ca(2+) before its increase.
Developmental Biology,
272 (2),
376-388.
Artículo
54 - Tatsu,Y., Nishigaki,T., Darszon,A., Yumoto,N. (2002). A caged sperm-activating peptide that has a photocleavable protecting group on the backbone amide.
FEBS Letters,
525 (1-3),
20-24.
Revisión
55 - Darszon,A., Beltran,C., Felix,R., Nishigaki,T., Trevino,C.L. (2001). Ion Transport in Sperm Signaling.
Developmental Biology,
240 (1),
1-14.
Artículo
56 - Nishigaki,T., Zamudio,F.Z., Possani,L.D., Darszon,A. (2001). Time-Resolved Sperm Responses to an Egg Peptide Measured by Stopped-Flow Fluorometry.
Biochemical and Biophysical Research Communications,
284 (2),
531-535.
Artículo
57 - Galindo,B.E., Nishigaki,T., Rodriguez,E., Sanchez,D., Beltran,C., Darszon,A. (2000). Speract-receptor interaction and the modulation of ion transport in Strongylocentrotus purpuratus sea urchin sperm.
Zygote,
8 (Suppl 1),
S20-S21.
Artículo; Actas Paper
58 - Hoshi,M., Nishigaki,T., Kawamura,M., Ikeda,M., Gunaratne,J., Ueno,S., Ogiso,M., Moriyama,H., Matsumoto,M. (2000). Acrosome reaction in starfish: signal molecules in the jelly coat and their receptors.
Zygote,
8 (Suppl 1),
S26-S27.
*
Artículo
59 - Nishigaki,T., Chiba,K., Hoshi,M. (2000). A 130-kDa membrane protein of sperm flagella is the receptor for asterosaps, sperm-activating peptides of starfish Asterias amurensis.
Developmental Biology,
219 (1),
154-162.
*
Artículo
60 - Nishigaki,T., Darszon,A. (2000). Real-time measurements of the interactions between fluorescent speract and its sperm receptor.
Developmental Biology,
223 (1),
17-26.
Revisión
61 - Darszon,A., Labarca,P., Nishigaki,T., Espinosa,F. (1999). Ion channels in sperm physiology.
Physiological Reviews,
79 (2),
481-510.
Artículo
62 - Matsumoto,M., Briones,A.V., Nishigaki,T., Hoshi,M. (1999). Sequence analysis of cDNAs encoding precursors of starfish asterosaps.
Developmental Genetics,
25 (2),
130-136.
*
Artículo
63 - Byrne,M., Cerra,A., Nishigaki,T., Hoshi,M. (1997). Infestation of the testes of the Japanese sea star Asterias amurensis by the ciliate Orchitophyra stellarum: A caution against the use of this ciliate for biological control.
Diseases Of Aquatic Organisms,
28 (3),
235-239.
*
Artículo
64 - Nishigaki,T., Chiba,K., Miki,W., Hoshi,M. (1996). Structure and function of asterosaps, sperm-activating peptides from the jelly coat of starfish eggs.
Zygote,
4 (3),
237-245.
*
Revisión
65 - Hoshi,M., Nishigaki,T., Ushiyama,A., Okinaga,T., Chiba,K., Matsumoto,M. (1994). Egg-jelly signal molecules for triggering the acrosome reaction in starfish spermatozoa.
International Journal of Developmental Biology,
38 (2),
167-174.
*
Internacional
66 - Nishigaki,T., Gonzalez-Cota,A.L., Orta Salazar,G.J. (2014). CatSper in Male Infertility. Pathologies of Calcium Channels.
713-728,
Berlin, Heidelberg: Springer.
Internacional
67 - Nishigaki,T., Trevino,C.L. (2012). Study of protein-protein interactions by fluorescence spectroscopy. Tools to Understand Protein-Protein Interactions.
87-100,
Kerala: Research Signpost.
Revisión
68 - Darszon,A., Trevino,C.L., Wood,C., Galindo,B., Rodriguez-Miranda,E., Acevedo,J.J., Hernandez-Gonzalez,E.O., Beltran,C., Martinez-Lopez,P., Nishigaki,T. (2007). Ion channels in sperm motility and capacitation. Society of Reproduction and Fertility supplement.
229-244,
65.
Memoria in extenso
69 - Byrne,M, Cerra,A., Nishigaki,T., Hoshi,M. (1998). Male infertility in Asterias amurensis: a new phenomenon resulting from introduction of the parasitic ciliate Orchitophyra stellarum to Japan. IX International Echiderm Conference.
203-207,
San Francisco, California.
*
Preprint
70 - Novero,A.G., Torres-Rodriguez,P., de la Vega-Beltran,J.L., Schiavi-Ehrenhaus,L.J., Luque,G.M., Carruba,M., Stival,C., Gentile,I., Ritagliati,C., Santi,C.M., Nishigaki,T., Krapf,D., Buffone,M.G., Darszon,A., Trevino,C.L., Krapf,D. (2024). The sodium-proton exchangers sNHE and NHE1 control plasma membrane hyperpolarization in mouse sperm.
bioRxiv,
Preprint posted March 6.
Preprint
71 - Garza-Lopez,E., Aldana,A., Darszon,A., Nishigaki,T., Lopez-Gonzalez,I. (2019). CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in their domains I/II.
bioRxiv,
Posted May 02, 2019,
626069.
Preprint
72 - Priego-Espinosa,D.A., Darszon,A., Guerrero,A., Gonzalez-Cota,A.L., Nishigaki,T., Martinez-Mekler,G., Carneiro,J. (2018). Modular mathematical analysis of the control of flagellar Ca 2+ -spike trains produced by CatSper and Ca v channels in sea urchin sperm.
bioRxiv,
preprint posted October 18, 2018.
Revisión
73 - Chavez,J.C., Carrasquel-Martinez,G., Hernandez-Garduno,S., Matamoros-Volante,A., Trevino,C.L., Nishigaki,T., Darszon,A.. Cytosolic and Acrosomal pH Regulation in Mammalian Sperm.
Cells,
13 (10),
865.
Artículo
74 - Pacheco-Castillo,H., Zagal-Huerta,E.E., Acevedo-Fernandez,J.J., Negrete-Leon,E., Nishigaki,T., Beltran,C.. Hyperglycemia adversely affects critical physiological events related to rat sperm capacitation.
Biochemical and Biophysical Research Communications,
734,
150610.
Artículo
75 - Sanchez-Guevara,Y., Oliver,E.I., Nishigaki,T.. Ca2+ concentrations in mouse sperm mitochondria fluctuate according to the cytosol.
Reproduction,
167 (2),
e230237.
Preprint
76 - Novero,A.G., Torres-Rodriguez,P., de la Vega-Beltran,J.L., Schiavi-Ehrenhaus,L.J., Luque,G.M., Carruba,M., Stival,C., Gentile,I., Ritagliati,C., Santi,C.M., Nishigaki,T., Krapf,D., Buffone,M.G., Darszon,A., Trevino,C.L., Krapf,D.. The sodium-proton exchangers sNHE and NHE1 control plasma membrane hyperpolarization in mouse sperm.
bioRxiv,
Preprint posted March 6.
Artículo
77 - Arcos-Hernandez,C., Suarez-Delgado,E., Islas,L.D., Romero,F., Lopez-Gonzalez,I., Ai,H.W., Nishigaki,T.. How to study a highly toxic protein to bacteria: A case of voltage sensor domain of mouse sperm-specific sodium/proton exchanger.
Protein Expression and Purification,
201,
106172.
Revisión
78 - Arcos-Hernandez,C., Nishigaki,T.. Ion currents through the voltage sensor domain of distinct families of proteins.
Journal of Biological Physics,
49,
393-413.
Artículo
Artículo
80 - Chavez,J.C., Darszon,A., Trevino,C.L., Nishigaki,T.. Quantitative intracellular pH determinations in single live mammalian spermatozoa using the ratiometric dye SNARF-5F.
Frontiers in Cell and Developmental Biology,
7,
366.
Revisión
81 - Darszon,A., Nishigaki,T., Lopez-Gonzalez,I., Visconti,P.E., Trevino,C.L.. Differences and Similarities: The Richness of Comparative Sperm Physiology.
Physiology (Bethesda.),
35 (3),
196-208.
Artículo
82 - De La Rosa,J.A.M., Garcia-Castaneda,M., Nishigaki,T., Gomora,J.C., Mancilla-Percino,T., Avila,G.. Interaction of MDIMP with the voltage-gated calcium channels.
Molecular Pharmacology,
98 (3),
211-221.
Artículo
83 - Garza-Lopez,E., Aldana,A., Darszon,A., Nishigaki,T., Lopez-Gonzalez,I.. CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in its domains I/II.
Cell Calcium,
89,
102214.
Artículo
84 - Priego-Espinosa,D.A., Darszon,A., Guerrero,A., Gonzalez-Cota,A.L., Nishigaki,T., Martinez-Mekler,G., Carneiro,J.. Modular analysis of the control of flagellar Ca2+-spike trains produced by CatSper and CaV channels in sea urchin sperm.
PLoS Computational Biology,
16 (3),
e1007605.
Artículo
85 - Kinoshita-Terauchi, N., Shiba, K., Terauchi, M., Romero, F., Ramirez-Gomez, H. V., Yoshida, M., Motomura, T., Kawai, H., Nishigaki, T.. High potassium seawater inhibits ascidian sperm chemotaxis, but does not affect the male gamete chemotaxis of a brown alga.
Zygote,
27 (4),
225-231.
Artículo
86 - Romero,F., Nishigaki,T.. Comparative genomic analysis suggests that the sperm-specific sodium/proton exchanger and soluble adenylyl cyclase are key regulators of CatSper among the Metazoa.
Zoological Letters,
5,
25.
Preprint
87 - Garza-Lopez,E., Aldana,A., Darszon,A., Nishigaki,T., Lopez-Gonzalez,I.. CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in their domains I/II.
bioRxiv,
Posted May 02, 2019,
626069.
Artículo
88 - Chavez, J.C., De la Vega-Beltran JL, Jose, O., Torres, P., Nishigaki, T., Trevino, C.L., Darszon, A.. Acrosomal alkalization triggers Ca2+ release and acrosome reaction in mammalian spermatozoa.
Journal of Cellular Physiology,
233 (6),
4735-4747.
Artículo
89 - Santana-Calvo, C., Romero, F., Lopez-Gonzalez, I., Nishigaki, T.. Robust evaluation of intermolecular FRET using a large Stokes shift fluorophore as a donor.
Biotechniques,
65 (4),
211-218.
Preprint
90 - Priego-Espinosa,D.A., Darszon,A., Guerrero,A., Gonzalez-Cota,A.L., Nishigaki,T., Martinez-Mekler,G., Carneiro,J.. Modular mathematical analysis of the control of flagellar Ca 2+ -spike trains produced by CatSper and Ca v channels in sea urchin sperm.
bioRxiv,
preprint posted October 18, 2018.
Artículo
91 - Romero, F., Santana-Calvo, C., Sanchez-Guevara, Y., Nishigaki, T.. FRET-based binding assay between a fluorescent cAMP analogue and a cyclic nucleotide-binding domain tagged with a CFP.
FEBS Letters,
591 (18),
2869-2878.
Artículo
92 - Arcos-Hernandez,C., Romero,F., Sanchez-Guevara,Y., Beltran,C., Nishigaki,T.. FRET analysis using sperm-activating peptides tagged with fluorescent proteins reveals that ligand-binding sites exist as clusters.
Journal of Experimental Biology,
219 (Pt 4),
508-515.
Artículo
93 - Garza-Lopez,E., Chavez,J.C., Santana-Calvo,C., Lopez-Gonzalez,I., Nishigaki,T.. Cd2+ sensitivity and permeability of a low voltage-activated Ca2+ channel with CatSper-like selectivity filter.
Cell Calcium,
60 (1),
41-50.
Artículo
94 - Gijsbers,A., Nishigaki,T., Sanchez-Puig,N.. Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions.
Journal of Visualized Experiments JoVE,
116.
Artículo
95 - Nishigaki,T., Romero,F., Sanchez-Guevara,Y.. CatSper, el canal de Ca2+ que regula el batido flagelar del espermatozoide en eucariontes.
Revista Iberoamericana de Ciencias,
3 (3),
28-36.
Artículo
96 - Garza-Lopez,E., Sanchez-Carranza,O., Nishigaki,T., Lopez-Gonzalez,I.. Pharmacological identification of endogenous slo1 channel-á1 subunit complexes in CHO cells using three àKTx1 subfamily toxins.
International Journal of Pharmacy & Therapeutics,
6 (1),
11-24.
Revisión
97 - Beltran,C., Rodriguez-Miranda,E., Granados-Gonzalez,G., de De la Torre LG, Nishigaki,T., Darszon,A.. Zn induces hyperpolarization by activation of a K channel and increases intracellular Ca and pH in sea urchin spermatozoa.
Developmental Biology,
394 (1),
15-23.
Revisión
98 - Nishigaki,T., Jose,O., Gonzalez-Cota,A.L., Romero,F., Trevino,C.L., Darszon,A.. Intracellular pH in Sperm Physiology.
Biochemical and Biophysical Research Communications,
450 (3),
1149-1158.
Artículo
99 - Sanchez-Tusie,A.A., Vasudevan,S.R., Churchill,G.C., Nishigaki,T., Trevino,C.L.. Characterization of NAADP-Mediated Calcium Signaling in Human Spermatozoa.
Biochemical and Biophysical Research Communications,
443 (2),
531-536.
Internacional
100 - Nishigaki,T., Gonzalez-Cota,A.L., Orta Salazar,G.J.. CatSper in Male Infertility. Pathologies of Calcium Channels.
713-728,
Berlin, Heidelberg: Springer.
Artículo
101 - Servin-Vences,M.R., Tatsu,Y., Ando,H., Guerrero,A., Yumoto,N., Darszon,A., Nishigaki,T.. A caged progesterone analog alters intracellular Ca2+ and flagellar bending in human sperm.
Reproduction,
144 (1),
101-109.
Internacional
102 - Nishigaki,T., Trevino,C.L.. Study of protein-protein interactions by fluorescence spectroscopy. Tools to Understand Protein-Protein Interactions.
87-100,
Kerala: Research Signpost.
Artículo
103 - Chavez,J.C., de Blas,G.A., de la Vega-Beltran,J.L., Nishigaki,T., Chirinos,M., Gonzalez-Gonzalez,M.E., Larrea,F., Solis,A., Darszon,A., Trevino,C.L.. The opening of maitotoxin-sensitive calcium channels induces the acrosome reaction in human spermatozoa: differences from the zona pellucida.
Asian Journal of Andrology,
13,
159-165.
Revisión
104 - Darszon,A., Nishigaki,T., Beltran,C., Trevino,C.L.. Calcium Channels in the Development, Maturation, and Function of Spermatozoa.
Physiological Reviews,
91 (4),
1305-1355.
Artículo
105 - Guerrero,A., Wood,C.D., Nishigaki,T., Carneiro,J., Darszon,A.. Tuning sperm chemotaxis.
Biochemical Society Transactions,
38 (5),
1270-1274.
Artículo
106 - Guerrero,A., Nishigaki,T., Carneiro,J., Tatsu,Y., Wood,C.D., Darszon,A.. Tuning sperm chemotaxis by calcium burst timing.
Developmental Biology,
344 (1),
52-65.
Artículo
107 - Darszon,A., Guerrero,A., Galindo,B.E., Nishigaki,T., Wood,C.D.. Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility.
International Journal of Developmental Biology,
52 (5-6),
595-606.
Artículo
108 - de la Sancha,C.U., Martinez-Cadena,G., Lopez-Godinez,J., Castellano,L.E., Nishigaki,T., Darszon,A., Garcia-Soto,J.. Rho-kinase (ROCK) in sea urchin sperm: Its role in regulating the intracellular pH during the acrosome reaction.
Biochemical and Biophysical Research Communications,
364 (3),
470-475 [Corrigendum en Volume 366, Issue 2, 2008, Page 604].
Artículo
109 - Rangel-Mata,F., Mendez-Marquez,R., Martinez-Cadena,G., Lopez-Godinez,J., Nishigaki,T., Darszon,A., Garcia-Soto,J.. Rho, Rho-kinase, and the actin cytoskeleton regulate the Na(+)-H(+) exchanger in sea urchin eggs.
Biochemical and Biophysical Research Communications,
352 (1),
264-269.
Artículo
110 - Wood,C.D., Nishigaki,T., Tatsu,Y., Yumoto,N., Baba,S.A., Whitaker,M., Darszon,A.. Altering the speract-induced ion permeability changes that generate flagellar Ca(2+) spikes regulates their kinetics and sea urchin sperm motility.
Developmental Biology,
306 (2),
525-537.
Revisión
111 - Darszon,A., Trevino,C.L., Wood,C., Galindo,B., Rodriguez-Miranda,E., Acevedo,J.J., Hernandez-Gonzalez,E.O., Beltran,C., Martinez-Lopez,P., Nishigaki,T.. Ion channels in sperm motility and capacitation. Society of Reproduction and Fertility supplement.
229-244,
65.
Revisión
112 - Darszon,A., Acevedo,J.J., Galindo,B.E., Hernandez-Gonzalez,E.O., Nishigaki,T., Trevino,C.L., Wood,C., Beltran,C.. Sperm channel diversity and functional multiplicity.
Reproduction,
131 (6),
977-988.
Artículo
113 - Nishigaki,T., Wood,C.D., Shiba,K., Baba,S.A., Darszon,A.. Stroboscopic illumination using light-emitting diodes reduces phototoxicity in fluorescence cell imaging.
Biotechniques,
41 (2),
191-197.
Artículo
114 - Trevino,C.L., de la Vega-Beltran,J.L., Nishigaki,T., Felix,R., Darszon,A.. Maitotoxin potently promotes Ca(2+) influx in mouse spermatogenic cells and sperm, and induces the acrosome reaction.
Journal of Cellular Physiology,
206 (2),
449-456.
Revisión
115 - Darszon,A., Nishigaki,T., Wood,C., Trevino,C.L., Felix,R., Beltran,C.. Calcium channels and ca(2+) fluctuations in sperm physiology.
International Review of Cytology,
243,
79-172.
Artículo
116 - Shiba,K., Ohmuro,J., Mogami,Y., Nishigaki,T., Wood,C.D., Darszon,A., Tatsu,Y., Yumoto,N., Baba,S.A.. Sperm-activating Peptide induces asymmetric flagellar bending in sea urchin sperm.
Zoological Science,
22 (3),
293-299.
Artículo
117 - Wood,C.D., Nisihigaki,T., Furuta,T., Baba,S.A., Darszon,A.. Real-time analysis of the role of Ca2+ in flagellar movement and motility in single sea urchin sperm.
Journal of Cell Biology,
169 (5),
725-731 .
Revisión
118 - Darszon,A., Wood,C.D., Beltran,C., Sanchez,D., Rodriguez,E., Gorelik,J., Korchev,Y.E., Nishigaki,T.. Measuring ion fluxes in sperm.
Methods in Cell Biology,
74,
545-576.
Artículo
119 - Nishigaki,T., Wood,C.D., Tatsu,Y., Yumoto,N., Furuta,T., Elias,D., Shiba,K., Baba,S.A., Darszon,A.. A sea urchin egg jelly peptide induces a cGMP-mediated decrease in sperm intracellular Ca(2+) before its increase.
Developmental Biology,
272 (2),
376-388.
Artículo
120 - Tatsu,Y., Nishigaki,T., Darszon,A., Yumoto,N.. A caged sperm-activating peptide that has a photocleavable protecting group on the backbone amide.
FEBS Letters,
525 (1-3),
20-24.
Revisión
121 - Darszon,A., Beltran,C., Felix,R., Nishigaki,T., Trevino,C.L.. Ion Transport in Sperm Signaling.
Developmental Biology,
240 (1),
1-14.
Artículo
122 - Nishigaki,T., Zamudio,F.Z., Possani,L.D., Darszon,A.. Time-Resolved Sperm Responses to an Egg Peptide Measured by Stopped-Flow Fluorometry.
Biochemical and Biophysical Research Communications,
284 (2),
531-535.
Artículo
123 - Galindo,B.E., Nishigaki,T., Rodriguez,E., Sanchez,D., Beltran,C., Darszon,A.. Speract-receptor interaction and the modulation of ion transport in Strongylocentrotus purpuratus sea urchin sperm.
Zygote,
8 (Suppl 1),
S20-S21.
Artículo; Actas Paper
124 - Hoshi,M., Nishigaki,T., Kawamura,M., Ikeda,M., Gunaratne,J., Ueno,S., Ogiso,M., Moriyama,H., Matsumoto,M.. Acrosome reaction in starfish: signal molecules in the jelly coat and their receptors.
Zygote,
8 (Suppl 1),
S26-S27.
*
Artículo
125 - Nishigaki,T., Chiba,K., Hoshi,M.. A 130-kDa membrane protein of sperm flagella is the receptor for asterosaps, sperm-activating peptides of starfish Asterias amurensis.
Developmental Biology,
219 (1),
154-162.
*
Artículo
126 - Nishigaki,T., Darszon,A.. Real-time measurements of the interactions between fluorescent speract and its sperm receptor.
Developmental Biology,
223 (1),
17-26.
Revisión
127 - Darszon,A., Labarca,P., Nishigaki,T., Espinosa,F.. Ion channels in sperm physiology.
Physiological Reviews,
79 (2),
481-510.
Artículo
128 - Matsumoto,M., Briones,A.V., Nishigaki,T., Hoshi,M.. Sequence analysis of cDNAs encoding precursors of starfish asterosaps.
Developmental Genetics,
25 (2),
130-136.
*
Memoria in extenso
129 - Byrne,M, Cerra,A., Nishigaki,T., Hoshi,M.. Male infertility in Asterias amurensis: a new phenomenon resulting from introduction of the parasitic ciliate Orchitophyra stellarum to Japan. IX International Echiderm Conference.
203-207,
San Francisco, California.
*
Artículo
130 - Byrne,M., Cerra,A., Nishigaki,T., Hoshi,M.. Infestation of the testes of the Japanese sea star Asterias amurensis by the ciliate Orchitophyra stellarum: A caution against the use of this ciliate for biological control.
Diseases Of Aquatic Organisms,
28 (3),
235-239.
*
Artículo
131 - Nishigaki,T., Chiba,K., Miki,W., Hoshi,M.. Structure and function of asterosaps, sperm-activating peptides from the jelly coat of starfish eggs.
Zygote,
4 (3),
237-245.
*
Revisión
132 - Hoshi,M., Nishigaki,T., Ushiyama,A., Okinaga,T., Chiba,K., Matsumoto,M.. Egg-jelly signal molecules for triggering the acrosome reaction in starfish spermatozoa.
International Journal of Developmental Biology,
38 (2),
167-174.
*
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