08Ago - 2022
La exocitosis acrosomal espermática durante la fertilización: nuevas perspectivas
12:00 PM - 01:00 PM|Dr. Mariano Buffone|Instituto de Biología y medicina experimental, Conicet, Buenos Aires Argentina.|Invitado por: Dr. Alberto Darszon.
Seminario
Resumen
La investigación del Dr. Buffone se ha centrado en estudiar diferentes aspectos de la capacitación de los espermatozoides de mamíferos, con especial énfasis en la exocitosis acrosomal. Su grupo ha utilizado métodos de microscopía avanzada para generar imágenes in vivo de la exocitosis acrosomal en tiempo real en combinación con microscopía de súper resolución. También han realizado experimentos in vivo utilizando ratones transgénicos, al obtener imágenes de la exocitosis acrosomal de los espermatozoides dentro del tracto reproductivo femenino.
Actualizado 2022-08-03 20:56:05
Biomolecular condensates in cellular stress responses and disease.
Simon Alberti. Professor of Cellular Biochemistry. Technische Universität Dresden. Center for Molecular and Cellular Bioengineering (CMCB). Biotechnology Center (BIOTEC)
Sede: Auditorio "Dr. Francisco G. Bolívar Zapata" del IBt/UNAM
01-Abril-2024 al 01-Abril-2024
05:00 PM
Dr. Rafael Martinez Gallegos
05:00 PM
Dr. Rafael Martinez Gallegos
Homologous recombination cloning: Tips, tricks and advantages
Using the endogenous homologous recombination of living organisms for cloning (HR-cloning) was first described in yeast and eventually in bacteria. Although not so widespread, this method allows the straightforward engineering of fusion proteins with the ability of exchanging elements from 1 nt to several kb within a plasmid. Therefore it can be used to manipulate codons or transcription factor target sequences as well as to study regulatory regions like full promoters or UTRs. Likewise, reporter markers can be exchanged having either N- or C-terminal variants. It also offers the possibility of engineering customized plasmids providing a different strategy to overcome difficulties in the cloning and editing of challenging DNA fragments. The manipulation steps are minimized and any recA1 bacteria strain is suitable for it. This method has been tested with common and customized plasmids with a range up to 15 kb. Its simplicity allows the implementation in any molecular biology laboratory without the requirement of any commercial Kit. I will discuss examples of HR-cloning on a wide variety of organisms, from plants to Drosophila.