Instituto de Biotecnologia UNAM

IBT-UNAM : Dr. Guillermo Gosset Lagarda


Dr. Guillermo Gosset Lagarda

Líder Académico »» Grupo


Tutor de Maestría y Doctorado

Nivel III del SNI

Departamento de Ingeniería Celular y Biocatálisis

Líneas de investigación



Oficina +52 (777) 329-1648
desde el D.F. 562-27648

red UNAM 27648
+52 (777) 311-4900 ext 217

Laboratorio +52 (777) 329-1601
desde el D.F. 562-27601

red UNAM 27601
+52 (777) 311-4900 ext 217

- Licenciatura: Biología, Escuela de Biología, Guadalajara, Jal. (1987)
- Maestría: Investigacion Biomedica Basica, CEINGEBI-UNAM (1988)
- Doctorado: en Biotecnología, IBt-UNAM (1993)
- Mencion honorífica por examen de Maestría (1988)
- Medalla "Gabino Barreda" por mejor promedio en estudios de Maestría (1989)


Q.B.P. Juan Carlos Fragoso

M.C. Laura Grecia Fuentes

David Ferreira

I.B.Q. Rene Rami­rez

Biol. Francisco Cruz

Maria Alejandra Mejia

Jose Manuel Camacho

Biol. Francisco Cruz

Biol. Miguel Atl Silva

Prisciluis Caheri Salas

Jesus Alberto Salgado

Ing. Fabian Perez

Publicaciones recientes

Carmona,S.B. Moreno,F. Bolivar,F. Gosset,G. Escalante,A. 2015. Inactivation of the PTS as a Strategy to Engineer the Production of Aromatic Metabolites in Escherichia coli Journal of Molecular Microbiology and Biotechnology, 25, 195-208.

Vargas-Tah,A. Martinez,L. Hernandez-Chavez,G. Rocha,M. Martinez,A. Bolivar,F. Gosset,G. 2015. Production of cinnamic and p -hydroxycinnamic acid from sugar mixtures with engineered Escherichia coli Microbial Cell Factories, 14, 6.

Balderas-Hernandez,V.E. Trevino-Quintanilla,L.G. Hernandez-Chavez,G. Martinez,A. Bolivar,F. Gosset,G. 2014. Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1 Microbial Cell Factories, 13, 136.

Rodriguez,A. Martinez,J.A. Flores,N. Escalante,A. Gosset,G. Bolivar,F. 2014. Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds Microbial Cell Factories, 13, 126.

Munoz-Gutierrez,I. Moss-Acosta,C. Trujillo-Martinez,B. Gosset,G. Martinez,A. 2014. Ag43-mediated display of a thermostable Microbial Cell Factories, 13, 106.

Ruiz-Villafan,B. Rodriguez-Sanoja,R. Aguilar-Osorio,G. Gosset,G. Sanchez,S. 2014. Glucose kinases from Streptomyces peucetius var. caesius Applied Microbiology and Biotechnology, 98, 6061-6071.

Cortes-Tolalpa,L. Gutierrez-Rios,R.M. Martinez,L.M. de Anda R. Gosset,G. Bolivar,F. Escalante,A. 2014. Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium Microbial Cell Factories, 13, 28.

Centeno-Leija,S. Huerta-Beristain,G. Giles-Gomez,M. Bolivar,F. Gosset,G. Martinez,A. 2014. Improving poly-3-hydroxybutyrate production in Escherichia coli by combining the increase in the NADPH pool and acetyl-CoA availability Antonie Van Leeuwenhoek, 105, 687-696.

Sabido,A. Sigala,J.C. Hernandez-Chavez,G. Flores,N. Gosset,G. Bolivar,F. 2014. Physiological and transcriptional characterization of Escherichia coli strains lacking interconversion of phosphoenolpyruvate and pyruvate when glucose and acetate are coutilized Biotechnology and Bioengineering, 111, 1150-1160.

Licona-Cassani,C. Lara,A.R. Cabrera-Valladares,N. Escalante,A. Hernandez-Chavez,G. Martinez,A. Bolivar,F. Gosset,G. 2014. Inactivation of Pyruvate Kinase or the Phosphoenolpyruvate: Sugar Phosphotransferase System Increases Shikimic and Dehydroshikimic Acid Yields from Glucose in Bacillus subtilis Journal of Molecular Microbiology and Biotechnology, 24, 37-45.

Wunderlich,M. Taymaz-Nikerel,H. Gosset,G. Ramirez,O.T. Lara,A.R. 2014. Effect of growth rate on plasmid DNA production and metabolic performance of engineered Escherichia coli strains Journal of Bioscience and Bioengineering, 117, 336-342.

Chavez-Bejar,M.I. Balderas-Hernandez,V.E. Gutierrez-Alejandre,A. Martinez,A. Bolivar,F. Gosset,G. 2013. Metabolic engineering of Escherichia coli to optimize melanin synthesis from glucose Microbial Cell Factories, 12, 108.

Rodriguez,A. Martinez,J.A. Baez-Viveros,J.L. Flores,N. Hernandez-Chavez,G. Ramirez,O.T. Gosset,G. Bolivar,F. 2013. Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS and pykF Microbial Cell Factories, 12, 86.

Centeno-Leija,S. Utrilla,J. Flores,N. Rodriguez,A. Gosset,G. Martinez,A. 2013. Metabolic and transcriptional response of Escherichia coli with a NADP-dependent glyceraldehyde 3-phosphate dehydrogenase from Streptococcus mutans Antonie Van Leeuwenhoek, 104, 913-924.

Fuentes,L.G. Lara,A.R. Martinez,L.M. Ramirez,O.T. Martinez,A. Bolivar,F. Gosset,G. 2013. Modification of glucose import capacity in Escherichia coli: physiologic consequences and utility for improving DNA vaccine production Microbial Cell Factories, 12, 42.

Soria,S. de Anda R. Flores,N. Romero-Garcia,S. Gosset,G. Bolivar,F. Baez-Viveros,J.L. 2013. New insights on transcriptional responses of genes involved in carbon central metabolism, respiration and fermentation to low ATP levels in Escherichia coli Journal of Basic Microbiology, 53, 365-380.

Sabido,A. Martinez,L.M. de Anda R. Martinez,A. Bolivar,F. Gosset,G. 2013. A novel plasmid vector designed for chromosomal gene integration and expression: Use for developing a genetically stable Escherichia coli melanin production strain Plasmid, 69, 16-23.

Borja,G.M. Meza-Mora E. Barron,B. Gosset,G. Ramirez,O.T. Lara,A.R. 2012. Engineering Escherichia coli to increase plasmid DNA production in high cell-density cultivations in batch mode Microbial Cell Factories, 11, 132.

Meza,E. Becker,J. Bolivar,F. Gosset,G. Wittmann,C. 2012. Consequences of phosphoenolpyruvate:sugar phosphotranferase system and pyruvate kinase isozymes inactivation in central carbon metabolism flux distribution in Escherichia coli Microbial Cell Factories, 11, 127.

Utrilla,J. Licona-Cassani,C. Marcellin,E. Gosset,G. Nielsen,L.K. Martinez,A. 2012. Engineering and adaptive evolution of Escherichia coli for d-lactate fermentation reveals GatC as a xylose transporter Metabolic Engineering, 14, 469-476.

Aguilar,C. Escalante,A. Flores,N. de Anda R. Riveros-McKay,F. Gosset,G. Morett,E. Bolivar,F. 2012. Genetic changes during a laboratory adaptive evolution process that allowed fast growth in glucose to an Escherichia coli strain lacking the major glucose transport system BMC Genomics, 13, 385.

Cabrera-Valladares,N. Martinez,L.M. Flores,N. Hernandez-Chavez,G. Martinez,A. Bolivar,F. Gosset,G. 2012. Physiologic Consequences of Glucose Transport and Phosphoenolpyruvate Node Modifications in Bacillus subtilis 168 Journal of Molecular Microbiology and Biotechnology, 22, 177-197.

Fernandez-Sandoval,M.T. Huerta-Beristain,G. Trujillo-Martinez,B. Bustos,P. Gonzalez,V. Bolivar,F. Gosset,G. Martinez,A. 2012. Laboratory metabolic evolution improves acetate tolerance and growth on acetate of ethanologenic Escherichia coli under non-aerated conditions in glucose-mineral medium Applied Microbiology and Biotechnology, 96, 1291-1300.

Munoz-Gutierrez,I. Oropeza,R. Gosset,G. Martinez,A. 2012. Cell surface display of a beta-glucosidase employing the type V secretion system on ethanologenic Escherichia coli for the fermentation of cellobiose to ethanol Journal of Industrial Microbiology & Biotechnology, 39, 1141-1152.

Chavez-Bejar,M.I. Baez-Viveros,J.L. Martinez,A. Bolivar,F. Gosset,G. 2012. Biotechnological production of l-tyrosine and derived compounds Process Biochemistry, 47, 1017-1026.

Escalante,A. Salinas-Cervantes A. Gosset,G. Bolivar,F. 2012. Current knowledge of the Escherichia coli phosphoenolpyruvate-carbohydrate phosphotransferase system: peculiarities of regulation and impact on growth and product formation Applied Microbiology and Biotechnology, 94, 1483-1494.

Martinez-Gomez,K. Flores,N. Castaneda,H.M. Martinez-Batallar,G. Hernandez-Chavez,G. Ramirez,O.T. Gosset,G. Encarnacion,S. Bolivar,F. 2012. New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol Microbial Cell Factories, 11, 46.

Pablos,T.E. Soto,R. Mora,E.M. LeBorgne S. Ramirez,O.T. Gosset,G. Lara,A.R. 2012. Enhanced production of plasmid DNA by engineered Escherichia coli strains Journal of Biotechnology, 158, 211-214.

Soto,R. Caspeta,L. Barron,B. Gosset,G. Ramirez,O.T. Lara,A.R. 2011. High cell-density cultivation in batch mode for plasmid DNA production by a metabolically engineered E. coli strain with minimized overflow metabolism Biochemical Engineering Journal, 56, 165-171.

Lopes,M.S.G. Gosset,G. Rocha,R.C.S. Gomez,J.G.C. Ferreira da Silva,L. 2011. PHB Biosynthesis in Catabolite Repression Mutant of Burkholderia sacchari Current Microbiology, 63, 319-326.

Pablos,T.E. Mora,E.M. Le Borgne S. Ramirez,O.T. Gosset,G. Lara,A.R. 2011. Vitreoscilla hemoglobin expression in engineered Escherichia coli: Improved performance in high cell-density batch cultivations Biotechnology Journal, 6, 993-1002.

Munoz,A.J. Hernandez-Chavez,G. de Anda R. Martinez,A. Bolivar,F. Gosset,G. 2011. Metabolic engineering of Escherichia coli for improving L: -3,4-dihydroxyphenylalanine (L: -DOPA) synthesis from glucose Journal of Industrial Microbiology & Biotechnology, 38, 1845-1852.

Tierrafria,V.H. Ramos-Aboites,H.E. Gosset,G. Barona-Gomez,F. 2011. Disruption of the siderophore-binding desE receptor gene in Streptomyces coelicolor A3(2) results in impaired growth in spite of multiple iron-siderophore transport systems Microbial Biotechnology, 4, 275-285.

Balderas-Hernandez,V.E. Hernandez-Montalvo,V. Bolivar,F. Gosset,G. Martinez,A. 2011. Adaptive Evolution of Escherichia coli Inactivated in the Phosphotransferase System Operon Improves Co-utilization of Xylose and Glucose Under Anaerobic Conditions Applied Biochemistry and Biotechnology, 163, 485-496.

Libros y capítulos recientes

Escalante-Lozada,A. Giles-Gomez,M. Gosset-Lagarda,G. 2014. El pulque, una bebida histórica con importantes implicaciones biotecnológicas en: Francisco Rebolledo Un Mundo de Bacterias. UNAM, Campus Morelos. pags. 91-95


Sabido,A. Martinez,A. Bolivar,F. Gosset,G. 2011. Generación de un Sistema para la Integración y Expresión de Genes en el Cromosoma de Escherichia coli Biotecnologia, 15, 35-49.

Cortes-Tolalpa,L. Carmona,S.B. Cervantes-Salinas,A. Garcia,M.A. Gosset,G. Escalante,A. Bolivar,F. 2011. Ingeniería de vías metabólicas en Escherichia coli para la producción de Shikimato como precursos para la síntesis de compuestos antivirales contra influenza. Biotecnologia, 15, 30-47.


A. Martínez J. G. Gosset L. G. T. Hernández Ch. G. Huerta B. B. Trujillo y M., J. Utrilla C. 2013 Strains of Escherichia coli modified by metabolic engineering to produce chemical compounds from hydrolyzed lignocellulose, pentoses, hexoses or other carbon sources.UNAM EUA 8563283, México No. 31474 y 3127021.

Ponce Laura Grecia , Martínez Mejía Luz María , Ramírez Reivich Octavio Tonatiuh , Martínez Jiménez Alfredo , Bolívar Zapata Francisco Gonzalo , Gosset Lagarda Guillermo . 2013 Cepas de Escherichia coli con alta capacidad para la producción de plásmidos y su uso en la producción de vacunas de ADN.UNAM México. (en trámite)

Guillermo Gosset Lagarda , Alfredo Martínez Jiménez , Francisco Gonzalo Bolívar Zapata , Ana Joyce Muñoz Arellano, Georgina Teresa Hernández Chávez y Ramón de Anda Herrera 2011 Cepas de Escherichia coli modificadas para producir 3,4-hidroxi-L-fenilalanina (L-Dopa).UNAM México. (en trámite)

J. Utrilla C. , Luz María Martínez Mejía G. Gosset L. A. Martínez J. 2011 Nuevos transportadores de xilosa y sus aplicaciones.UNAM México. (en trámite)

A. R. Lara R., M. de C. Vázquez L., G. Gosset L., F. G. Bolívar Z., A. Lopez-Munguia C. y O. T. Ramírez R 2006 Estrategia para generar células insensibles a condiciones heterogéneas en biorreactores industriales a través de mutaciones de vías metabólicas anaerobias.UNAM México. (en trámite)

J. Osuna, J.L. Báez, G. Hernández, F. G. Bolivar, X. Soberón y G. Gosset 2005 Versiones insensibles a inhibición alostérica y catalíticamente eficientes de la enzima corismato mutasa-prefenato deshidratasa y su aplicación para la producción de L-fenilalanina en microorganismos. México.

G. Gosset, A. Martínez, F. G. Bolívar, V. H. Lagunas, N. Cabrera, J. G. Dávila, V. M. González y V. P. Bustos 2004 Producción de melaninas en microorganismos recombinantes.UNAM México. (en trámite)

F. G. Bolívar Z. G. Gosset L. R. de Anda R. Quintero R. A. Martínez F. Valle N. Flores M. 1994 Proceso fermentativo para obtener proteínas híbridas a partir de cepas de Escherichia coli.UNAM México.

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