. Instituto de Biotecnologia UNAM

IBT-UNAM : Dr. Alberto Darszon Israel

Dr. Alberto Darszon Israel

Líder Académico »» Grupo
Investigador
Tutor de Maestría y Doctorado
Nivel Inv. de Excelencia del SNI
Departamento de Genética del Desarrollo y Fisiología Molecular

Líneas de investigación

email: darszon @ibt.unam.mx

- Licenciatura: Química, Universidad Iberoamericana, Fac. de Química (1972)
- Doctorado: en Ciencias, CINVESTAV-IPN, Dpto. de Bioquímica, (1977)
- Estancia de Investigación: Universidad de California, en San Diego, CA, E.U.A. (1978)

Premio Scopus México 2008 Editorial Elsevier (2008)
Premio Imaging Solutions of the Year, en el área de Microscopía Revista Industrial Advanced Imaging (2008)
Wellcome Trust Collaborative Research Initiative Grant 2004-2007 (2004)
Fogarty International Research Collaboration Award (2003-2005) NIH (2003)
Premio Universidad Nacional en el área de investigación en Ciencias Naturales UNAM (2000)
Howard Hughes Medical Institute International Research Scholar 1991-1996 (1991)
Premio Miguel Alemán en el Area Salud (1989)

Estudiantes

M.C. Gabriela Carrasquel

Verónica Loyo

M.C. Hector Vicente Ramirez

Publicaciones recientes

Gadelha,H. Hernandez-Herrera,P. Montoya,F. Darszon,A. Corkidi,G. 2020. Human sperm uses asymmetric and anisotropic flagellar controls to regulate swimming symmetry and cell steering Science Advances, 6, eaba5168.

Garza-Lopez,E. Aldana,A. Darszon,A. Nishigaki,T. Lopez-Gonzalez,I. 2020. CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in its domains I/II Cell Calcium, 89, 102214.

Darszon,A. Nishigaki,T. Lopez-Gonzalez,I. Visconti,P.E. Trevino,C.L. 2020. Differences and Similarities: The Richness of Comparative Sperm Physiology Physiology (Bethesda.), 35, 196-208.

Ramirez-Gomez,H.V. Jimenez-Sabinina,V. Velazquez-Perez M. Beltran,C. Carneiro,J. Wood,C.D. Tuval,I. Darszon,A. Guerrero,A. 2020. Sperm chemotaxis is driven by the slope of the chemoattractant concentration field Elife, 9, e50532.

Priego-Espinosa,D.A. Darszon,A. Guerrero,A. Gonzalez-Cota,A.L. Nishigaki,T. Martinez-Mekler,G. Carneiro,J. 2020. Modular analysis of the control of flagellar Ca2+-spike trains produced by CatSper and CaV channels in sea urchin sperm PLoS Computational Biology, 16, e1007605.

Chavez,J.C. Darszon,A. Trevino,C.L. Nishigaki,T. 2020. Quantitative intracellular pH determinations in single live mammalian spermatozoa using the ratiometric dye SNARF-5F Frontiers in Cell and Developmental Biology, 7, 366.

Navarrete,F.A. Aguila,L. Martin-Hidalgo,D. Tourzani,D.A. Luque,G.M. Ardestani,G. Garcia-Vazquez,F.A. Levin,L.R. Buck,J. Darszon,A. Buffone,M.G. Mager,J. Fissore,R.A. Salicioni,A.M. Gervasi,M.G. Visconti,P.E. 2019. Transient Sperm Starvation Improves the Outcome of Assisted Reproductive Technologies Frontiers in Cell and Developmental Biology, 7, 262.

Bondarenko,O. Corzo,G. Santana,F.L. del Rio-Portilla,F. Darszon,A. Lopez-Gonzalez,I. 2019. Non-enzymatically oxidized arachidonic acid regulates T-type Ca(2+) currents in mouse spermatogenic cells FEBS Letters, 593, 1735-1750 [Preprint anteriormente en biorXiv].

Bastian-Eugenio,C.E. Bohorquez-Hernandez,A. Pacheco,J. Sampieri,A. Asanov,A. Ocelotl-Oviedo,J.P. Guerrero,A. Darszon,A. Vaca,L. 2019. Heterologous calcium-dependent inactivation of Orai1 by neighboring TRPV1 channels modulates cell migration and wound healing Communications Biology, 2, 88.

Brukman,N.G. Nunez,S.Y. Puga Molina,L.D.C. Buffone,M.G. Darszon,A. Cuasnicu,P.S. Da Ros,V. 2019. Tyrosine phosphorylation signaling regulates Ca(2+) entry by affecting intracellular pH during human sperm capacitation Journal of Cellular Physiology, 234, 5276-5288.

Zhao,R. Kennedy,K. de Blas,G.A. Orta,G. Pavarotti,M.A. Arias,R.J. de la Vega-Beltran,J.L. Li,Q. Dai,H. Perozo,E. Mayorga,L.S. Darszon,A. Goldstein,S.A.N. 2018. Role of human Hv1 channels in sperm capacitation and white blood cell respiratory burst established by a designed peptide inhibitor Proceedings of the National Academy of Sciences of the United States of America, 115, E11847-E11856.

Romarowski,A. Velasco Felix,A.G. Torres-Rodriguez P. Gervasi,M.G. Xu,X. Luque,G.M. Contreras-Jimenez,G. Sanchez-Cardenas,C. Ramirez-Gomez,H.V. Krapf,D. Visconti,P.E. Krapf,D. Guerrero,A. Darszon,A. Buffone,M.G. 2018. Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis Journal of Cell Science, 131, jcs218958.

Orta,G. Vega-Beltran,J.L. Hidalgo,D. Santi,C.M. Visconti,P. Darszon,A. 2018. CatSper channels are regulated by protein kinase A Journal of Biological Chemistry, 293, 16830.

Sanchez-Cardenas,C. Montoya,F. Navarrete,F.A. Hernandez-Cruz,A. Corkidi,G. Visconti,P.E. Darszon,A. 2018. Intracellular Ca2+ threshold reversibly switches flagellar beat off and on Biology of Reproduction, 99, 1010-1021.

Hernandez-Herrera,P. Montoya,F. Rendon-Mancha,J.M. Darszon,A. Corkidi,G. 2018. 3D+t Human Sperm Flagellum Tracing in low SNR Fluorescence Images IEEE Transactions on Medical Imaging, 37, 2236-2247.

Puga Molina,L.C. Pinto,N.A. Torres,N.I. Gonzalez-Cota,A.L. Luque,G.M. Balestrini,P.A. Romarowski,A. Krapf,D. Santi,C.M. Trevino,C.L. Darszon,A. Buffone,M.G. 2018. CFTR/ENaC dependent regulation of membrane potential during human sperm capacitation is initiated by bicarbonate uptake through NBC Journal of Biological Chemistry, 293, 9924-9936.

Stival,C. Ritagliati,C. Xu,X. Gervasi,M.G. Luque,G.M. Baro,G.C. Vega-Beltran,J.L. Torres,N.I. Darszon,A. Krapf,D. Buffone,M.G. Visconti,P. Krapf,D. 2018. Disruption of protein kinase A localization induces acrosomal exocytosis in capacitated mouse sperm Journal of Biological Chemistry, 293, 9435-9447.

Mata-Martinez,E. Darszon,A. Trevino,C.L. 2018. pH-dependent Ca(+2) oscillations prevent untimely acrosome reaction in human sperm Biochemical and Biophysical Research Communications, 497, 146-152.

Chavez,J.C. de la Vega-Beltran,J.L. Jose,O. Torres,P. Nishigaki,T. Trevino,C.L. Darszon,A. 2018. Acrosomal alkalization triggers Ca2+ release and acrosome reaction in mammalian spermatozoa Journal of Cellular Physiology, 233, 4735-4747.

Corkidi,G. Montoya,F. Hernandez-Herrera,P. Rios-Herrera,W.A. Muller,M.F. Trevino,C.L. Darszon,A. 2017. Are there intracellular Ca2+ oscillations correlated with flagellar beating in human sperm? A three vs. two-dimensional analysis Molecular Human Reproduction, 23, 583-593.

Espinal-Enriquez,J. Priego-Espinosa,D.A. Darszon,A. Beltran,C. Martinez-Mekler,G. 2017. Network model predicts that CatSper is the main Ca2+ channel in the regulation of sea urchin sperm motility Scientific Reports, 7, 4236.

Puga Molina,L.C. Pinto,N.A. Torres-Rodriguez P. Romarowski,A. Vicens-Sanchez A. Visconti,P.E. Darszon,A. Trevino,C.L. Buffone,M.G. 2017. Essential Role of CFTR in PKA-Dependent Phosphorylation, Alkalinization and Hyperpolarization During Human Sperm Capacitation Journal of Cellular Physiology, 232, 1404-1414.

Navarrete,F.A. Alvau,A. Lee,H.C. Levin,L.R. Buck,J. Leon,P.M. Santi,C.M. Krapf,D. Mager,J. Fissore,R.A. Salicioni,A.M. Darszon,A. Visconti,P.E. 2016. Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models Scientific Reports, 6, 33589.

Alvau,A. Battistone,M.A. Gervasi,M.G. Navarrete,F.A. Xu,X. Sanchez-Cardenas,C. de la Vega-Beltran,J.L. Da- Ros,V. Greer,P. Darszon,A. Krapf,D. Salicioni,A.M. Cuasnicu,P. Visconti,P.E. 2016. The tyrosine kinase FER is responsible for the capacitation-associated increase in tyrosine phosphorylation in murine sperm Development, 143, 2325-2333.

Romarowski,A. Sanchez-Cardenas,C. Ramirez-Gomez,H.V. Puga Molina,L.D. Trevino,C.L. Hernandez,C.A. Darszon,A. Buffone,M.G. 2016. A Specific Transitory Increase in Intracellular Calcium Induced by Progesterone Promotes Acrosomal Exocytosis in Mouse Sperm Biology of Reproduction, 94, 63.

Garcia-Rincon,J. Darszon,A. Beltran,C. 2016. Speract, a sea urchin egg peptide that regulates sperm motility, also stimulates sperm mitochondrial metabolism Biochimica et Biophysica Acta (BBA), 1857, 415-426.

Lopez-Gonzalez,I. Trevino,C.L. Darszon,A. 2016. Regulation of Spermatogenic Cell T-Type Ca Currents by Zn : Implications in Male Reproductive Physiology Journal of Cellular Physiology, 231, 659-667.

Ernesto,J.I. Weigel-Munoz M. Battistone,M.A. Vasen,G. Martinez-Lopez,P. Orta,G. Figueiras-Fierro,D. de la Vega-Beltran,J.L. Moreno,I.A. Guidobaldi,H.A. Giojalas,L. Darszon,A. Cohen,D.J. Cuasnicu,P.S. 2015. CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization Journal of Cell Biology, 210, 1213-1224.

Sanchez-Carranza,O. Torres-Rodriguez,P. Darszon,A. Trevino,C.L. Lopez-Gonzalez,I. 2015. Pharmacology of hSlo3 channels and their contribution in the capacitation-associated hyperpolarization of human sperm Biochemical and Biophysical Research Communications, 466, 554-559.

Gonzalez-Cota,A.L. Silva,P.A. Carneiro,J. Darszon,A. 2015. Single cell imaging reveals that the motility regulator speract induces a flagellar alkalinization that precedes and is independent of Ca(2+) influx in sea urchin spermatozoa FEBS Letters, 589, 2146-2154.

Escoffier,J. Navarrete,F. Haddad,D. Santi,C.M. Darszon,A. Visconti,P.E. 2015. Flow Cytometry Analysis Reveals That Only a Subpopulation of Mouse Sperm Undergoes Hyperpolarization During Capacitation Biology of Reproduction, 92, 121.

Navarrete,F.A. Garcia-Vazquez,F.A. Alvau,A. Escoffier,J. Krapf,D. Sanchez-Cardenas,C. Salicioni,A.M. Darszon,A. Visconti,P.E. 2015. Biphasic Role of Calcium in Mouse Sperm Capacitation Signaling Pathways Journal of Cellular Physiology, 230, 1758-1769.

Chavez,J.C. Ferreira,G.J. Butler,A. Trevino,C.L. Darszon,A. Salkoff,L. Santi,C.M. 2014. SLO3 K+ Channels Control Calcium Entry through CATSPER Channels in Sperm Journal of Biological Chemistry, 289, 32266-32275.

Espinal-Enriquez,J. Darszon,A. Guerrero,A. Martinez-Mekler,G. 2014. In Silico Determination of the Effect of Multi-Target Drugs on Calcium Dynamics Signaling Network Underlying Sea Urchin Spermatozoa Motility PLoS ONE, 9, e104451.

Sanchez-Cardenas,C. Servin-Vences,M.R. Jose,O. Trevino,C.L. Hernandez-Cruz,A. Darszon,A. 2014. Acrosome Reaction and Ca2+ Imaging in Single Human Spermatozoa: New Regulatory Roles of [Ca2+]i Biology of Reproduction, 91, 67.

Beltran,C. Rodriguez-Miranda,E. Granados-Gonzalez,G. de De la Torre LG Nishigaki,T. Darszon,A. 2014. Zn induces hyperpolarization by activation of a K channel and increases intracellular Ca and pH in sea urchin spermatozoa Developmental Biology, 394, 15-23.

Vacquier,V.D. Loza-Huerta,A. Garcia-Rincon,J. Darszon,A. Beltran,C. 2014. Soluble adenylyl cyclase of sea urchin spermatozoa Biochimica et Biophysica Acta (BBA), 1842, 2621-2628.

Nishigaki,T. Jose,O. Gonzalez-Cota,A.L. Romero,F. Trevino,C.L. Darszon,A. 2014. Intracellular pH in Sperm Physiology Biochemical and Biophysical Research Communications, 450, 1149-1158.

Lopez-Gonzalez,I. Torres-Rodriguez,P. Sanchez-Carranza,O. Solis-Lopez,A. Santi,C.M. Darszon,A. Trevino,C.L. 2014. Membrane Hyperpolarization during Human Sperm Capacitation Molecular Human Reproduction, 20, 619-629.

Darszon,A. Hernandez-Cruz,A. 2014. T-type Ca channels in spermatogenic cells and sperm Pflugers Archiv European Journal of Physiology, 466, 819-831.

Tateno,H. Krapf,D. Hino,T. Sanchez-Cardenas,C. Darszon,A. Yanagimachi,R. Visconti,P.E. 2013. Ca2+ ionophore A23187 can make mouse spermatozoa capable of fertilizing in vitro without activation of cAMP-dependent phosphorylation pathways Proceedings of the National Academy of Sciences of the United States of America, 110, 18543-18548.

Wertheimer,E. Krapf,D. Vega-Beltran,J.L. Sanchez-Cardenas,C. Navarrete,F. Haddad,D. Escoffier,J. Salicioni,A.M. Levin,L.R. Buck,J. Mager,J. Darszon,A. Visconti,P.E. 2013. Compartmentalization of Distinct cAMP Signaling Pathways in Mammalian Sperm Journal of Biological Chemistry, 288, 35307-35320.

Loza-Huerta,A. Vera-Estrella,R. Darszon,A. Beltran,C. 2013. Certain Strongylocentrotus purpuratus sperm mitochondrial proteins co-purify with low density detergent-insoluble membranes and are PKA or PKC-substrates possibly involved in sperm motility regulation Biochimica et Biophysica Acta (BBA), 1830, 5305-5315.

Balderas,E. Sanchez-Cardenas,C. Chavez,J.C. de la Vega-Beltran,J.L. Gomez-Lagunas,F. Trevino,C.L. Darszon,A. 2013. The anti-inflammatory drug celecoxib inhibits t-type Ca currents in spermatogenic cells yet it elicits the acrosome reaction in mature sperm FEBS Letters, 587, 2412-2419.

Chavez,J.C. de la Vega-Beltran,J.L. Escoffier,J. Visconti,P.E. Trevino,C.L. Darszon,A. Salkoff,L. Santi,C.M. 2013. Ion Permeabilities in Mouse Sperm Reveal an External Trigger for SLO3-Dependent Hyperpolarization PLoS ONE, 8, e60578.

Guerrero,A. Espinal,J. Wood,C.D. Rendon,J.M. Carneiro,J. Martinez-Mekler,G. Darszon,A. 2013. Niflumic acid disrupts marine spermatozoan chemotaxis without impairing the spatiotemporal detection of chemoattractant gradients Journal of Cell Science, 126, 1477-1487.

Santi,C.M. Orta,G. Salkoff,L. Visconti,P.E. Darszon,A. Trevino,C.L. 2013. K(+) and cl(-) channels and transporters in sperm function Current Topics in Developmental Biology, 102, 385-421.

Figueiras-Fierro,D. Acevedo,J.J. Martinez-Lopez,P. Escoffier,J. Sepulveda,F.V. Balderas,E. Orta,G. Visconti,P. Darszon,A. 2013. Electrophysiological evidence for the presence of cystic fibrosis transmembrane conductance regulator (CFTR) in mouse sperm Journal of Cellular Physiology, 228, 590-601.

de la Vega-Beltran,J.L. Sanchez-Cardenas,C. Krapf,D. Hernandez-Gonzalez,E.O. Wertheimer,E. Trevino,C.L. Visconti,P.E. Darszon,A. 2012. Mouse sperm membrane potential hyperpolarization is necessary and sufficient to prepare sperm for the acrosome reaction Journal of Biological Chemistry, 287, 44384-44393.

Sanchez-Cardenas,C. Guerrero,A. Trevino,C.L. Hernandez-Cruz,A. Darszon,A. 2012. Acute Slices of Mice Testis Seminiferous Tubules Unveil Spontaneous and Synchronous Ca2+ Oscillations in Germ Cell Clusters Biology of Reproduction, 87, 92.

Servin-Vences,M.R. Tatsu,Y. Ando,H. Guerrero,A. Yumoto,N. Darszon,A. Nishigaki,T. 2012. A caged progesterone analog alters intracellular Ca2+ and flagellar bending in human sperm Reproduction, 144, 101-109.

Darszon,A. Sanchez-Cardenas,C. Orta,G. Sanchez-Tusie,A.A. Beltran,C. Lopez-Gonzalez,I. Granados-Gonzalez,G. Trevino,C.L. 2012. Are TRP channels involved in sperm development and function? Cell and Tissue Research, 349, 749-764.

Orta,G. Ferreira,G. Jose,O. Trevino,C.L. Beltran,C. Darszon,A. 2012. Human spermatozoa possess a calcium-dependent chloride channel that may participate in the acrosomal reaction Journal of Physiology, 590, 2659-2675.

Escoffier,J. Krapf,D. Navarrete,F. Darszon,A. Visconti,P.E. 2012. Flow cytometry analysis reveals a decrease in intracellular sodium during sperm capacitation Journal of Cell Science, 125, 473-485.

Pimentel,J.A. Carneiro,J. Darszon,A. Corkidi,G. 2012. A segmentation algorithm for automated tracking of fast swimming unlabelled cells in three dimensions Journal of Microscopy, 245, 72-81.

Chavez,J.C. Hernandez-Gonzalez,E.O. Wertheimer,E. Visconti,P.E. Darszon,A. Trevino,C.L. 2012. Participation of the Cl-/HCO3- Exchangers SLC26A3 and SLC26A6, the Cl- Channel CFTR and the Regulatory Factor SLC9A3R1 in Mouse Sperm Capacitation Biology of Reproduction, 86, 1-14.

Balderas,E. Arteaga-Tlecuitl,R. Rivera,M. Gomora,J. Darszon,A. 2012. Niflumic acid blocks native and recombinant T-type channels Journal of Cellular Physiology, 227, 2542-255n.

Galindo,B.E. Darszon,A. 2011. Calmodulin antagonists inhibit sea urchin sperm hyperpolarization necessary for directed movement toward the egg.Proceedings of the Western Pharmacology Society, 54, 80-82.

Darszon,A. Nishigaki,T. Beltran,C. Trevino,C.L. 2011. Calcium Channels in the Development, Maturation, and Function of Spermatozoa Physiological Reviews, 91, 1305-1355.

Espinal,J. Aldana,M. Guerrero,A. Wood,C. Darszon,A. Martinez-Mekler,G. 2011. Discrete dynamics model for the speract-activated ca signaling network relevant to sperm motility PLoS ONE, 6, e22619.

Torres-Flores,V. Picazo-Juarez,G. Hernandez-Rueda,Y. Darszon,A. Gonzalez-Martinez,M.T. 2011. Sodium influx induced by external calcium chelation decreases human sperm motility Human Reproduction, 26, 2626-2635.

Guerrero,A. Carneiro,J. Pimentel,A. Wood,C.D. Corkidi,G. Darszon,A. 2011. Strategies for locating the female gamete: the importance of measuring sperm trajectories in three spatial dimensions Molecular Human Reproduction, 17, 511-523.

Visconti,P.E. Krapf,D. de la Vega-Beltran,J.L. Acevedo,J.J. Darszon,A. 2011. Ion channels, phosphorylation and mammalian sperm capacitation Asian Journal of Andrology, 13, 395-405.

Gibbs,G.M. Orta,G. Reddy,T. Koppers,A.J. Martinez-Lopez,P. de la Vega-Beltran,J.L. Lo,J.C. Veldhuis,N. Jamsai,D. McIntyre,P. Darszon,A. O'Bryan,M.K. 2011. Cysteine-rich secretory protein 4 is an inhibitor of transient receptor potential M8 with a role in establishing sperm function Proceedings of the National Academy of Sciences of the United States of America, 108, 7034-7039.

Martinez-Lopez,P. Trevino,C.L. de la Vega-Beltran,J.L. Blas,G.D. Monroy,E. Beltran,C. Orta,G. Gibbs,G.M. O'Bryan,M.K. Darszon,A. 2011. TRPM8 in mouse sperm detects temperature changes and may influence the acrosome reaction Journal of Cellular Physiology, 226, 1620-1631.

Chavez,J.C. de Blas,G.A. de la Vega-Beltran,J.L. Nishigaki,T. Chirinos,M. Gonzalez-Gonzalez,M.E. Larrea,F. Solis,A. Darszon,A. Trevino,C.L. 2011. The opening of maitotoxin-sensitive calcium channels induces the acrosome reaction in human spermatozoa: differences from the zona pellucida Asian Journal of Andrology, 13, 159-165.

Guerrero,A. Wood,C.D. Nishigaki,T. Carneiro,J. Darszon,A. 2010. Tuning sperm chemotaxis Biochemical Society Transactions, 38, 1270-1274.

Reyes,J.G. Osses,N. Knox,M. Darszon,A. Trevino,C.L. 2010. Glucose and lactate regulate maitotoxin-activated Ca2+ entry in spermatogenic cells: the role of intracellular [Ca2+] FEBS Letters, 584, 3111-3115.

Guerrero,A. Nishigaki,T. Carneiro,J. Tatsu,Y. Wood,C.D. Darszon,A. 2010. Tuning sperm chemotaxis by calcium burst timing Developmental Biology, 344, 52-65.

Jose,O. Hernandez-Hernandez,O. Chirinos,M. Gonzalez-Gonzalez,M.E. Larrea,F. Almanza,A. Felix,R. Darszon,A. Trevino,C.L. 2010. Recombinant human ZP3-induced sperm acrosome reaction: Evidence for the involvement of T- and L-type voltage-gated calcium channels Biochemical and Biophysical Research Communications, 395, 530-534.

Suhaiman,L. de Blas,G.A. Obeid,L.M. Darszon,A. Mayorga,L.S. Belmonte,S.A. 2010. Sphingosine 1-phosphate and sphingosine kinase are involved in a novel signaling pathway leading to acrosomal exocytosis Journal of Biological Chemistry, 285, 16302-16314.

Santi,C.M. Martinez-Lopez,P. de la Vega-Beltran,J.L. Butler,A. Alisio,A. Darszon,A. Salkoff,L. 2010. The SLO3 sperm-specific potassium channel plays a vital role in male fertility FEBS Letters, 584, 1041-1046.

de Blas,G.A. Darszon,A. Ocampo,A.Y. Serrano,C.J. Castellano,L.E. Hernandez-Gonzalez,E.O. Chirinos,M. Larrea,F. Beltran,C. Trevino,C.L. 2009. TRPM8, a versatile channel in human sperm PLoS ONE, 4, e6095.

Branham,M.T. Bustos,M.A. de Blas,G.A. Rehmann,H. Zarelli,V.E. Trevino,C.L. Darszon,A. Mayorga,L.S. Tomes,C.N. 2009. Epac activates the small G proteins Rap1 and Rab3A to achieve exocytosis Journal of Biological Chemistry, 284, 24825-24839.

Martinez-Lopez,P. Santi,C.M. Trevino,C.L. Ocampo-Gutierrez,A.Y. Acevedo,J.J. Alisio,A. Salkoff,L.B. Darszon,A. 2009. Mouse sperm K+ currents stimulated by pH and cAMP possibly coded by Slo3 channels Biochemical and Biophysical Research Communications, 381, 204-209.

Ardon,F. Rodriguez-Miranda,E. Beltran,C. Hernandez-Cruz,A. Darszon,A. 2009. Mitochondrial inhibitors activate influx of external Ca(2+) in sea urchin sperm Biochimica et Biophysica Acta (BBA), 1787, 15-24.

Wertheimer,E.V. Salicioni,A.M. Liu,W. Trevino,C.L. Chavez,J. Hernandez-Gonzalez,E.O. Darszon,A. Visconti,P.E. 2008. Chloride is essential for capacitation and for the capacitation-associated increase in tyrosine phosphorylation Journal of Biological Chemistry, 283, 35539-35550.

Darszon,A. Guerrero,A. Galindo,B.E. Nishigaki,T. Wood,C.D. 2008. Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility International Journal of Developmental Biology, 52, 595-606.

Corkidi,G. Taboada,B. Wood,C.D. Guerrero,A. Darszon,A. 2008. Tracking sperm in three-dimensions Biochemical and Biophysical Research Communications, 373, 125-129.

de la Sancha,C.U. Martinez-Cadena,G. Lopez-Godinez,J. Castellano,L.E. Nishigaki,T. Darszon,A. Garcia-Soto,J. 2007. Rho-kinase (ROCK) in sea urchin sperm: Its role in regulating the intracellular pH during the acrosome reaction Biochemical and Biophysical Research Communications, 364, 470-475 [Corrigendum en Volume 366, Issue 2, 2008, Page 604].

Darszon,A. Trevino,C.L. Wood,C. Galindo,B. Rodriguez-Miranda,E. Acevedo,J.J. Hernandez-Gonzalez,E.O. Beltran,C. Martinez-Lopez,P. Nishigaki,T. 2007. Ion channels in sperm motility and capacitation.Society of Reproduction and Fertility supplement, 65, 229-244.

Hernandez-Gonzalez,E.O. Trevino,C.L. Castellano,L.E. de la Vega-Beltran,J.L. Ocampo,A.Y. Wertheimer,E. Visconti,P.E. Darszon,A. 2007. Involvement of cystic fibrosis transmembrane conductance regulator in mouse sperm capacitation Journal of Biological Chemistry, 282, 24397-24406.

Beltran,C. Vacquier,V.D. Moy,G. Chen,Y. Buck,J. Levin,L.R. Darszon,A. 2007. Particulate and soluble adenylyl cyclases participate in the sperm acrosome reaction Biochemical and Biophysical Research Communications, 358, 1128-1135.

Wood,C.D. Nishigaki,T. Tatsu,Y. Yumoto,N. Baba,S.A. Whitaker,M. Darszon,A. 2007. Altering the speract-induced ion permeability changes that generate flagellar Ca(2+) spikes regulates their kinetics and sea urchin sperm motility Developmental Biology, 306, 525-537.

Castillo,E. Lopez-Gonzalez,I. De Regil-Hernandez,R. Reyes-Duarte,D. Sanchez-Herrera,D. Lopez-Munguia,A. Darszon,A. 2007. Enzymatic synthesis of capsaicin analogs and their effect on the T-type Ca(2+) channels Biochemical and Biophysical Research Communications, 356, 424-430.

Galindo,B.E. de la Vega-Beltran,J.L. Labarca,P. Vacquier,V.D. Darszon,A. 2007. Sp-tetraKCNG: A novel cyclic nucleotide gated K(+) channel Biochemical and Biophysical Research Communications, 354, 668-675.

Rangel-Mata,F. Mendez-Marquez,R. Martinez-Cadena,G. Lopez-Godinez,J. Nishigaki,T. Darszon,A. Garcia-Soto,J. 2007. Rho, Rho-kinase, and the actin cytoskeleton regulate the Na(+)-H(+) exchanger in sea urchin eggs Biochemical and Biophysical Research Communications, 352, 264-269.

Munoz-Garay,C. Sanchez,J. Darszon,A. de Maagd,R.A. Bakker,P. Soberon,M. Bravo,A. 2006. Permeability Changes of Manduca sexta Midgut Brush Border Membranes Induced by Oligomeric Structures of Different Cry Toxins Journal of Membrane Biology, 212, 61-68 [Erratum: 245 (12) 859].

Nishigaki,T. Wood,C.D. Shiba,K. Baba,S.A. Darszon,A. 2006. Stroboscopic illumination using light-emitting diodes reduces phototoxicity in fluorescence cell imaging Biotechniques, 41, 191-197.

Gasque,G. Labarca,P. Delgado,R. Darszon,A. 2006. Bridging behavior and physiology: Ion-channel perspective on mushroom body-dependent olfactory learning and memory in Drosophila Journal of Cellular Physiology, 209, 1046-1053.

Darszon,A. Lopez-Martinez,P. Acevedo,J.J. Hernandez-Cruz,A. Trevino,C.L. 2006. T-type Ca(2+) channels in sperm function Cell Calcium, 40, 241-252.

Darszon,A. Acevedo,J.J. Galindo,B.E. Hernandez-Gonzalez,E.O. Nishigaki,T. Trevino,C.L. Wood,C. Beltran,C. 2006. Sperm channel diversity and functional multiplicity Reproduction, 131, 977-988.

Hernandez-Gonzalez,E.O. Sosnik,J. Edwards,J. Acevedo,J.J. Mendoza-Lujambio,I. Lopez-Gonzalez,I. Demarco,I. Wertheimer,E. Darszon,A. Visconti,P.E. 2006. Sodium and epithelial sodium channels participate in the regulation of the capacitation-associated hyperpolarization in mouse sperm Journal of Biological Chemistry, 281, 5623-5633.

Acevedo,J.J. Mendoza-Lujambio,I. de la Vega-Beltran,J.L. Trevino,C.L. Felix,R. Darszon,A. 2006. K(ATP) channels in mouse spermatogenic cells and sperm, and their role in capacitation Developmental Biology, 289, 395-405.

Trevino,C.L. de la Vega-Beltran,J.L. Nishigaki,T. Felix,R. Darszon,A. 2006. Maitotoxin potently promotes Ca(2+) influx in mouse spermatogenic cells and sperm, and induces the acrosome reaction Journal of Cellular Physiology, 206, 449-456.

Granados-Gonzalez,G. Mendoza-Lujambio,I. Rodriguez,E. Galindo,B.E. Beltran,C. Darszon,A. 2005. Identification of voltage-dependent Ca(2+) channels in sea urchin sperm FEBS Letters, 579, 6667-6672.

Gasque,G. Labarca,P. Darszon,A. 2005. Cholesterol-depleting compounds modulate K(+)-currents in Drosophila Kenyon cells FEBS Letters, 579, 5129-5134.

Nomura,M. Beltran,C. Darszon,A. Vacquier,V.D. 2005. A soluble adenylyl cyclase from sea urchin spermatozoa Gene, 353, 231-238.

Wood,C.D. Nisihigaki,T. Furuta,T. Baba,S.A. Darszon,A. 2005. Real-time analysis of the role of Ca2+ in flagellar movement and motility in single sea urchin sperm Journal of Cell Biology, 169, 725-731 [correction J Cell Biol 170 (7): 1171-1171 SEP 26 2005].

Darszon,A. Nishigaki,T. Wood,C. Trevino,C.L. Felix,R. Beltran,C. 2005. Calcium channels and ca(2+) fluctuations in sperm physiology International Review of Cytology, 243, 79-172.

Shiba,K. Ohmuro,J. Mogami,Y. Nishigaki,T. Wood,C.D. Darszon,A. Tatsu,Y. Yumoto,N. Baba,S.A. 2005. Sperm-activating Peptide induces asymmetric flagellar bending in sea urchin sperm Zoological Science, 22, 293-299.

Gasque,G. Labarca,P. Reynaud,E. Darszon,A. 2005. Shal and shaker differential contribution to the k+ currents in the Drosophila mushroom body neurons Journal of Neuroscience, 25, 2348-2358.

Darszon,A. Wood,C.D. Beltran,C. Sanchez,D. Rodriguez,E. Gorelik,J. Korchev,Y.E. Nishigaki,T. 2004. Measuring ion fluxes in sperm Methods in Cell Biology, 74, 545-576.

Schulz,J.R. de la Vega-Beltran,J.L. Beltran,C. Vacquier,V.D. Darszon,A. 2004. Ion channel activity of membrane vesicles released from sea urchin sperm during the acrosome reaction Biochemical and Biophysical Research Communications, 321, 88-93.

Nishigaki,T. Wood,C.D. Tatsu,Y. Yumoto,N. Furuta,T. Elias,D. Shiba,K. Baba,S.A. Darszon,A. 2004. A sea urchin egg jelly peptide induces a cGMP-mediated decrease in sperm intracellular Ca(2+) before its increase Developmental Biology, 272, 376-388.

Trevino,C.L. Felix,R. Castellano,L.E. Gutierrez,C. Rodriguez,D. Pacheco,J. Lopez-Gonzalez,I. Gomora,J.C. Tsutsumi,V. Hernandez-Cruz,A. Fiordelisio,T. Scaling,A.L. Darszon,A. 2004. Expression and differential cell distribution of low-threshold Ca(2+) channels in mammalian male germ cells and sperm FEBS Letters, 563, 87-92.

Felix,R. Sandoval,A. Sanchez,D. Gomora,J.C. Vega-Beltran,J.L. Trevino,C.L. Darszon,A. 2003. ZD7288 inhibits low-threshold Ca(2+) channel activity and regulates sperm function Biochemical and Biophysical Research Communications, 311, 187-192.

Wood,C.D. Darszon,A. Whitaker,M. 2003. Speract induces calcium oscillations in the sperm tail Journal of Cell Biology, 161, 89-101.

Castellano,L.E. Trevino,C.L. Rodriguez,D. Serrano,C.J. Pacheco,J. Tsutsumi,V. Felix,R. Darszon,A. 2003. Transient receptor potential (TRPC) channels in human sperm: expression, cellular localization and involvement in the regulation of flagellar motility FEBS Letters, 541, 69-74.

Lopez-Gonzalez,I. Olamendi-Portugal,T. de la Vega-Beltran,J.L. van der Walt,J. Dyason,K. Possani,L.D. Felix,R. Darszon,A. 2003. Scorpion toxins that block T-type Ca(2+) channels in spermatogenic cells inhibit the sperm acrosome reaction Biochemical and Biophysical Research Communications, 300, 408-414.

Rodriguez,E. Darszon,A. 2003. Intracellular sodium changes during the speract response and the acrosome reaction in sea urchin sperm Journal of Physiology, 546, 89-100.

Demarco,I.A. Espinosa,F. Edwards,J. Sosnik,J. de la Vega-Beltran,J.L. Hockensmith,J.W. Kopf,G.S. Darszon,A. Visconti,P.E. 2003. Involvement of a Na+/HCO3-cotransporter in mouse sperm capacitation Journal of Biological Chemistry, 278, 7001-7009.

Gorelik,J. Gu,Y. Spohr,H.A. Shevchuk,A.I. Lab,M.J. Harding,S.E. Edwards,C.R. Whitaker,M. Moss,G.W. Benton,D.C. Sanchez,D. Darszon,A. Vodyanoy,I. Klenerman,D. Korchev,Y.E. 2002. Ion channels in small cells and subcellular structures can be studied with a smart patch-clamp system Biophysical Journal, 83, 3296-3303.

Olamendi-Portugal,T. Garcia,B.I. Lopez-Gonzalez,I. van der Walt,J. Dyason,K. Ulens,C. Tytgat,J. Felix,R. Darszon,A. Possani,L.D. 2002. Two new scorpion toxins that target voltage-gated Ca(2+) and Na(+) channels Biochemical and Biophysical Research Communications, 299, 562-568.

de Blas,G. Michaut,M. Trevino,C.L. Tomes,C.N. Yunes,R. Darszon,A. Mayorga,L.S. 2002. The intraacrosomal calcium pool plays a direct role in acrosomal exocytosis Journal of Biological Chemistry, 277, 49326-49331.

Tatsu,Y. Nishigaki,T. Darszon,A. Yumoto,N. 2002. A caged sperm-activating peptide that has a photocleavable protecting group on the backbone amide FEBS Letters, 525, 20-24.

Felix,R. Serrano,C.J. Trevino,C.L. Munoz-Garay,C. Bravo,A. Navarro,A. Pacheco,J. Tsutsumi,V. Darszon,A. 2002. Identification of distinct K+ channels in mouse spermatogenic cells and sperm Zygote, 10, 183-188.

Trevino,C.L. Serrano,C.J. Beltran,C. Felix,R. Darszon,A. 2001. Identification of mouse trp homologs and lipid rafts from spermatogenic cells and sperm FEBS Letters, 509, 119-125.

Darszon,A. Beltran,C. Felix,R. Nishigaki,T. Trevino,C.L. 2001. Ion Transport in Sperm Signaling Developmental Biology, 240, 1-14.

Lopez-Gonzalez,I. de la Vega-Beltran,J.L. Santi,C.M. Florman,H.M. Felix,R. Darszon,A. 2001. Calmodulin antagonists inhibit t-type ca(2+) currents in mouse spermatogenic cells and the zona pellucida-induced sperm acrosome reaction Developmental Biology, 236, 210-219.

Gonzalez-Martinez,M.T. Galindo,B.E. de la Torre,L. Zapata,O. Rodriguez,E. Florman,H.M. Darszon,A. 2001. A sustained increase in intracellular ca(2+) is required for the acrosome reaction in sea urchin sperm Developmental Biology, 236, 220-229.

Sanchez,D. Labarca,P. Darszon,A. 2001. Sea urchin sperm cation-selective channels directly modulated by cAMP FEBS Letters, 503, 111-115.

Nishigaki,T. Zamudio,F.Z. Possani,L.D. Darszon,A. 2001. Time-Resolved Sperm Responses to an Egg Peptide Measured by Stopped-Flow Fluorometry Biochemical and Biophysical Research Communications, 284, 531-535.

Munoz-Garay,C. de la Vega-Beltran,J.L. Delgado,R. Labarca,P. Felix,R. Darszon,A. 2001. Inwardly rectifying k(+) channels in spermatogenic cells: functional expression and implication in sperm capacitation Developmental Biology, 234, 261-274.

Nishigaki,T. Darszon,A. 2000. Real-time measurements of the interactions between fluorescent speract and its sperm receptor Developmental Biology, 223, 17-26.

Galindo,B.E. Nishigaki,T. Rodriguez,E. Sanchez,D. Beltran,C. Darszon,A. 2000. Speract-receptor interaction and the modulation of ion transport in Strongylocentrotus purpuratus sea urchin sperm Zygote, 8, S20-S21.

Galindo,B.E. Beltran,C. Cragoe,E.J. Darszon,A. 2000. Participation of a K(+) channel modulated directly by cGMP in the speract-induced signaling cascade of strongylocentrotus purpuratus sea urchin sperm Developmental Biology, 221, 285-294.

O'Toole,C.M. Arnoult,C. Darszon,A. Steinhardt,R.A. Florman,H.M. 2000. Ca(2+) entry through store-operated channels in mouse sperm is initiated by egg ZP3 and drives the acrosome reaction Molecular Biology of the Cell, 11, 1571-1584.

Espinosa,F. Lopez-Gonzalez,I. Munoz-Garay,C. Felix,R. de la Vega-Beltran,J.L. Kopf,G.S. Visconti,P.E. Darszon,A. 2000. Dual regulation of the T-type Ca(2+) current by serum albumin and beta-estradiol in mammalian spermatogenic cells FEBS Letters, 475, 251-256.

Srivastava,A. Darszon,A. Strasser,R.J. 2000. Influence of water on the primary photosynthetic activity of Rhodospirillum rubrum in reverse micelles Photosynthetica, 38, 333-341.

Darszon,A. Labarca,P. Nishigaki,T. Espinosa,F. 1999. Ion channels in sperm physiology Physiological Reviews, 79, 481-510.

Espinosa,F. Lopez-Gonzalez,I. Serrano,C.J. Gasque,G. de la Vega-Beltran,J.L. Trevino,C.L. Darszon,A. 1999. 3.0.CO;2-B> Anion channel blockers differentially affect T-type Ca(2+) currents of mouse spermatogenic cells, alpha1E currents expressed in Xenopus oocytes and the sperm acrosome reaction Developmental Genetics, 25, 103-114.

Serrano,C.J. Trevino,C.L. Felix,R. Darszon,A. 1999. Voltage-dependent Ca(2+) channel subunit expression and immunolocalization in mouse spermatogenic cells and sperm FEBS Letters, 462, 171-176.

Srivastava,A. Rivara-Minten,E. Obregon,C. Darszon,A. Strasser,R.J. 1999. The role of water on photochemical activities of membrane protein complexes of bacteria.Archives Des Sciences, 52, 17-27.

Srivastava,A. Darszon,A. Strasser,R.J. 1999. The influence of water on the stability and activity of photosynthetic complexes, membranes and cells in apolar systems.Archives Des Sciences, 52, 73-99.

Darszon,A. Espinoza,F. Santi,C. Lievano,A. Lopez,I. de la Vega-Beltran,J.L. Delgado,R. Labarca,P. Hernandez,A. 1998. Ca 2+ and Cl.Zygote, 6, S22-S23.

Martinez,F. Munoz-Garay,C. Gurrola,G. Darszon,A. Possani,L.D. Becerril,B. 1998. Site directed mutants of Noxiustoxin reveal specific interactions with potassium channels FEBS Letters, 429, 381-384.

Trevino,C.L. Santi,C.M. Beltran,C. Hernandez-Cruz,A. Darszon,A. Lomeli,H. 1998. Localisation of inositol trisphosphate and ryanodine receptors during mouse spermatogenesis: possible functional implications Zygote, 6, 159-172.

Guerrero,A. Garcia,L. Zapata,O. Rodriguez,E. Darszon,A. 1998. Acrosome reaction inactivation in sea urchin sperm Biochimica Et Biophysica Acta-Molecular Cell Research, 1401, 329-338.

Espinosa,F. de la Vega-Beltran,J.L. Lopez-Gonzalez,I. Delgado,R. Labarca,P. Darszon,A. 1998. Mouse sperm patch-clamp recordings reveal single Cl- channels sensitive to niflumic acid, a blocker of the sperm acrosome reaction FEBS Letters, 426, 47-51.

Lokuta,A.J. Darszon,A. Beltran,C. Valdivia,H.H. 1998. Detection and functional characterization of ryanodine receptors from sea urchin eggs Journal of Physiology, 510, 155-164.

Santi,C.M. Santos,T. Hernandez-Cruz,A. Darszon,A. 1998. Properties of a novel pH-dependent Ca2+ permeation pathway present in male germ cells with possible roles in spermatogenesis and mature sperm function Journal of General Physiology, 112, 33-53.

Lorence,A. Darszon,A. Bravo,A. 1997. Aminopeptidase dependent pore formation of Bacillus thuringiensis Cry1Ac toxin on Trichoplusia ni membranes FEBS Letters, 414, 303-307.

Zapata,O. Ralston,J. Beltran,C. Parys,J.B. Chen,J.L. Longo,F.J. Darszon,A. 1997. Inositol triphosphate receptors in sea urchin sperm Zygote, 5, 355-364.

Kernen,P. Agosti,R.D. Strasser,R.J. Darszon,A. 1997. ATPase activity of thylakoid membranes in CTAB-hexanol-octane low water system Biochimica Et Biophysica Acta-Bioenergetics, 1321, 71-78.

Dehesa-Davila,M. Ramirez,A.N. Zamudio,F.Z. Gurrola-Briones,G. Lievano,A. Darszon,A. Possani,L.D. 1996. Structural and functional comparison of toxins from the venom of the scorpions Centruroides infamatus infamatus, Centruroides limpidus limpidus and Centruroides noxius Comparative Biochemistry and Physiology, 113, 331-339.

Darszon,A. Lievano,A. Beltran,C. 1996. Ion channels: key elements in gamete signaling Current Topics in Developmental Biology, 34, 117-167.

Labarca,P. Santi,C. Zapata,O. Morales,E. Beltran,C. Lievano,A. Darszon,A. 1996. A cAMP regulated K+-selective channel from the sea urchin sperm plasma membrane Developmental Biology, 174, 271-280.

Beltran,C. Zapata,O. Darszon,A. 1996. Membrane potential regulates sea urchin sperm adenylylcyclase Biochemistry, 35, 7591-7598.

Lievano,A. Santi,C.M. Serrano,C.J. Trevino,C.L. Bellve,A.R. Hernandez-Cruz,A. Darszon,A. 1996. T-type Ca2+ channels and alpha1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction FEBS Letters, 388, 150-154.

Santi,C.M. Darszon,A. Hernandez-Cruz,A. 1996. A dihydropyridine-sensitive T-type Ca2+ current is the main Ca2+ current carrier in mouse primary spermatocytes American Journal of Physiology Cell Physiology, 271, C1583-C1593.

Sepulveda-Becerra,M.A. Ferreira,S.T. Strasser,R.J. Garzon-Rodriguez,W. Beltran,C. Gomez-Puyou,A. Darszon,A. 1996. Refolding of triosephosphate isomerase in low-water media investigated by fluorescence resonance energy transfer Biochemistry, 35, 15915-15922.

Fernandez-Velasco,D.A. Sepulveda-Becerra,M. Galina,A. Darszon,A. Tuena-de-Gomez-Puyou,M. Gomez-Puyou,A. 1995. Water requirements in monomer folding and dimerization of triosephosphate isomerase in reverse micelles. Intrinsic fluorescence of conformers related to reactivation Biochemistry, 34, 361-369.

Lorence,A. Darszon,A. Diaz,C. Lievano,A. Quintero,R. Bravo,A. 1995. Delta-endotoxins induce cation channels in Spodoptera frugiperda brush border membranes in suspension and in planar lipid bilayers FEBS Letters, 360, 217-222.

Castellano,L.E. Lopez-Godinez,J. Aldana,G. Barrios-Rodiles,M. Obregon,A. Garcia de la Torre,L. Darszon,A. Garcia-Soto,J. 1995. The acrosome reaction in digitonin-permeabilized sea urchin sperm in the absence of the natural inducer.European Journal of Cell Biology, 67, 23-31.

Labarca,P. Zapata,O. Beltran,C. Darszon,A. 1995. Ion channels from the mouse sperm plasma membrane in planar lipid bilayers Zygote, 3, 199-206.

Espinosa,F. Darszon,A. 1995. Mouse sperm membrane potential: changes induced by Ca2+ FEBS Letters, 372, 119-125.

Cuellar-Mata,P. Martinez-Cadena,G. Castellano,L.E. Aldana-Veloz,G. Novoa-Martinez,G. Vargas,I. Darszon,A. Garcia-Soto,J. 1995. Multiple GTP-binding proteins in sea urchin sperm: Evidence for Gs and small G-proteins Development Growth & Differentiation, 37, 173-181.

Sepulveda-Becerra,M.A. Ferreira,S.T. Strasser,R. Garzon-Rodriguez,W. Beltran,C. Gomez-Puyou,A. Darszon,A. 1995. REASSOCIATION OF TRIOSEPHOSPHATE ISOMERASE IN REVERSE MICELLES MEASURED BY ENERGY-TRANSFER.Protein Engineering, 8, 22-22.

Darszon,A. Labarca,P. Beltran,C. Garcia-Soto,J. Lievano,A. 1994. Sea urchin sperm: an ion channel reconstitution study case Methods, 6, 37-50.

Beltran,C. Darszon,A. Labarca,P. Lievano,A. 1994. A high-conductance voltage-dependent multistate Ca2+ channel found in sea urchin and mouse spermatozoa FEBS Letters, 338, 23-26.

Shoshani,L. Darszon,A. Tuena-de-Gomez-Puyou,M. Gomez-Puyou,A. 1994. Activity and fluorescence changes of lactate dehydrogenase induced by guanidine hydrochloride in reverse micelles European Journal of Biochemistry, 221, 1027-1032.

Reynaud,E. de la Torre,L. Zapata,O. Lievano,A. Darszon,A. 1993. Ionic bases of the membrane potential and intracellular pH changes induced by speract in swollen sea urchin sperm FEBS Letters, 329, 210-214.

Morales,E. de la Torre,L. Moy,G.W. Vacquier,V.D. Darszon,A. 1993. Anion channels in the sea urchin sperm plasma membrane Molecular Reproduction and Development, 36, 174-182.

Gonzalez-Martinez,M.T. Guerrero,A. Morales,E. de la Torre,L. Darszon,A. 1992. A depolarization can trigger Ca2+ uptake and the acrosome reaction when preceded by a hyperpolarization in L. pictus sea urchin sperm Developmental Biology, 150, 193-202.

Garza-Ramos,G. Fernandez-Velasco,D.A. Ramirez,L. Shoshani,L. Darszon,A. Tuena-de-Gomez-Puyou,M. Gomez-Puyou,A. 1992. Enzyme activation by denaturants in organic solvent systems with a low water content European Journal of Biochemistry, 205, 509-517.

Fernandez-Velasco,D.A. Garza-Ramos,G. Ramirez,L. Shoshani,L. Darszon,A. Tuena-de-Gomez-Puyou,M. Gomez-Puyou,A. 1992. Activity of heart and muscle lactate dehydrogenases in all-aqueous systems and in organic solvents with low amounts of water. Effect of guanidine chloride European Journal of Biochemistry, 205, 501-508.

Babcock,D.F. Bosma,M.M. Battaglia,D.E. Darszon,A. 1992. Early persistent activation of sperm K+ channels by the egg peptide speract Proceedings of the National Academy of Sciences of the United States of America, 89, 6001-6005.

Strasser,R.J. Millan,L. Darszon,A. 1992. Inner mitochondrial membranes bound to concanavalin A-sepharose display succinate dehydrogenase, ATPase, and cytochrome oxidase activity Biotechnology and Bioengineering, 39, 1080-1085.

Kernen,P. Darszon,A. Strasser,R.J. 1992. MACROCOMPLEXES OF PHOTOACTIVE MEMBRANES IN APOLAR MEDIA.Archives Des Sciences, 45, 85-97.

Garcia-Soto,J. Araiza,L.M. Barrios,M. Darszon,A. Luna-Arias,J.P. 1991. Endogenous activity of cyclic nucleotide-dependent protein kinase in plasma membranes isolated from Strongylocentrotus purpuratus sea urchin sperm Biochemical and Biophysical Research Communications, 180, 1436-1445.

Garza-Ramos,G. Darszon,A. Tuena-de-Gomez-Puyou,M. Gomez-Puyou,A. 1990. Enzyme catalysis in organic solvents with low water content at high temperatures. The adenosinetriphosphatase of submitochondrial particles Biochemistry, 29, 751-757 * .

Lievano,A. Vega-Saenz-de-Miera,E.C. Darszon,A. 1990. Ca2+ channels from the sea urchin sperm plasma membrane Journal of General Physiology, 95, 273-296 * .

Garza-Ramos,G. Darszon,A. Tuena-de-Gomez-Puyou,M. Gomez-Puyou,A. 1990. High concentrations of guanidine chloride activate lactate dehydrogenase in low water media Biochemical and Biophysical Research Communications, 172, 830-834 * .

Gomez-Lagunas,F. Pena,A. Lievano,A. Darszon,A. 1989. Incorporation of ionic channels from yeast plasma membranes into black lipid membranes Biophysical Journal, 56, 115-119 * .

Prestipino,G. Valdivia,H.H. Lievano,A. Darszon,A. Ramirez,A.N. Possani,L.D. 1989. Purification and reconstitution of potassium channel proteins from squid axon membranes FEBS Letters, 250, 570-574.

Guerrero,A. Darszon,A. 1989. Egg jelly triggers a calcium influx which inactivates and is inhibited by calmodulin antagonists in the sea urchin sperm Biochimica et Biophysica Acta (BBA), 980, 109-116 * .

Garza-Ramos,G. Darszon,A. Tuena-de-Gomez-Puyou,M. Gomez-Puyou,A. 1989. Catalysis and thermostability of mitochondrial F1-ATPase in toluene-phospholipid-low-water systems Biochemistry, 28, 3177-3182 * .

Escamilla,E. Ayala,G. de Gomez-Puyou,M.T. Gomez-Puyou,A. Millan,L. Darszon,A. 1989. Catalytic activity of cytochrome oxidase and cytochrome c in apolar solvents containing phospholipids and low amounts of water Archives of Biochemistry and Biophysics, 272, 332-343 * .

Guerrero,A. Darszon,A. 1989. Evidence for the activation of two different Ca2+ channels during the egg jelly-induced acrosome reaction of sea urchin sperm Journal of Biological Chemistry, 264, 19593-19599 * .

Darszon,A. Escamilla,E. Gomez-Puyou,A. Tuena-de-Gomez-Puyou,M. 1988. Transfer of spores, bacteria and yeast into toluene containing phospholipids and low amounts of water: preservation of the bacterial respiratory chain Biochemical and Biophysical Research Communications, 151, 1074-1080 * .

Garcia-Soto,J. Mourelle,M. Vargas,I. de la Torre,L. Ramirez,E. Lopez-Colome,A.M. Darszon,A. 1988. Sea urchin sperm head plasma membranes: characteristics and egg jelly induced Ca2+ and Na+ uptake Biochimica et Biophysica Acta (BBA), 944, 1-12 * .

Darszon,A. Guerrero,A. Lievano,A. Gonzalez-Martinez,M. Morales,E. 1988. IONIC CHANNELS IN SEA-URCHIN SPERM PHYSIOLOGY News In Physiological Sciences, 3, 181-185 * .

Garcia-Soto,J. Gonzalez-Martinez,M. de la Torre,L. Darszon,A. 1987. Internal pH can regulate Ca2+ uptake and the acrosome reaction in sea urchin sperm Developmental Biology, 120, 112-120 * .

Gonzalez-Martinez,M. Darszon,A. 1987. A fast transient hyperpolarization occurs during the sea urchin sperm acrosome reaction induced by egg jelly FEBS Letters, 218, 247-250 * .

Guerrero,A. Sanchez,J.A. Darszon,A. 1987. Single-channel activity in sea urchin sperm revealed by the patch-clamp technique FEBS Letters, 220, 295-298 * .

Darszon,A. Garciasoto,J. Gonzalez,M. Guerrero,A. Sanchez,J.A. Lievano,A. 1986. Ion-Transport Mechanisms in Sea-Urchin Sperm Development Growth & Differentiation, 28, 17-18 * .

Ayala,G. de Gomez-Puyou,M.T. Gomez-Puyou,A. Darszon,A. 1986. Thermostability of membrane enzymes in organic solvents FEBS Letters, 203, 41-43 * .

Beltran,C. Tuena-de-Gomez-Puyou,M. Darszon,A. Gomez-Puyou,A. 1986. Simultaneous synthesis and hydrolysis of ATP regulated by the inhibitor protein in submitochondrial particles European Journal of Biochemistry, 160, 163-168 * .

Garcia-Soto,J. de la Torre,L. Darszon,A. 1985. Increasing intracellular pH of sea urchin sperm with NH4Cl induces Ca2+ uptake and acrosome reaction in the absence of egg jelly FEBS Lett., 190, 33-36 * .

Garcia-Soto,J. Darszon,A. 1985. High pH-induced acrosome reaction and Ca2+ uptake in sea urchin sperm suspended in Na+-free seawater Developmental Biology, 110, 338-345 * .

Lievano,A. Sanchez,J.A. Darszon,A. 1985. Single-channel activity of bilayers derived from sea urchin sperm plasma membranes at the tip of a patch-clamp electrode Developmental Biology, 112, 253-257 * .

Ayala,G. Nascimento,A. Gomez-Puyou,A. Darszon,A. 1985. Extraction of mitochondrial membrane proteins into organic solvents in a functional state Biochimica et Biophysica Acta (BBA), 810, 115-122 * .

Mourelle,M. Vargas,I. Darszon,A. 1984. Adenylate cyclase activity of membrane fractions isolated from sea urchin sperm Gamete Research, 9, 87-97 * .

Tuena-de-Gomez-Puyou,M. Ayala,G. Darszon,A. Gomez-Puyou,A. 1984. Oxidative phosphorylation and the Pi-ATP exchange reaction of submitochondrial particles under the influence of organic solvents Journal of Biological Chemistry, 259, 9472-9478 * .

Beltran,C. de Gomez-Puyou,M.T. Gomez-Puyou,A. Darszon,A. 1984. Release of the inhibitory action of the natural ATPase inhibitor protein on the mitochondrial ATPase European Journal of Biochemistry, 144, 151-157 * .

Darszon,A. Gould,M. de la Torre,L. Vargas,I. 1984. Response of isolated sperm plasma membranes from sea urchin to egg jelly European Journal of Biochemistry, 144, 515-522 * .

Darszon,A. 1983. Strategies in the reassembly of membrane proteins into lipid bilayer systems and their functional assay Journal of Bioenergetics and Biomembranes, 15, 321-334 * .

Ramakrishnan,V.R. Darszon,A. Montal,M. 1983. A small angle x-ray scattering study of a rhodopsin-lipid complex in hexane Journal of Biological Chemistry, 258, 4857-4860 * .

Gomez-Puyou,A. Sanchez-Bustamante,V.J. Darszon,A. Tuena-de-Gomez-Puyou,M. 1982. Interaction of mitochondrial ATPase with its inhibitor protein Annals of the New York Academy of Sciences, 402, 164-166 * .

Darszon,A. Gomez-Puyou,A. 1982. Extraction of mitochondrial protein-lipid complexes into organic solvents: an approach to study the interaction between the ATPase and the mitochondrial ATPase-inhibitor protein European Journal of Biochemistry, 121, 427-433 * .

Darszon,A. 1982. Rhodopsin-phospholipid complexes in apolar solvents: characteristics and mechanism of extraction Vision Research, 22, 1443-1446 * .

Sanchez-Bustamante,V.J. Darszon,A. Gomez-Puyou,A. 1982. On the function of the natural ATPase inhibitor protein in intact mitochondria European Journal of Biochemistry, 126, 611-616 * .

Montal,M. Darszon,A. Schindler,H. 1981. Functional reassembly of membrane proteins in planar lipid bilayers Quarterly Reviews of Biophysics, 14, 1-79 * .

Barranco,J. Darszon,A. Gomez-Puyou,A. 1981. Extraction of mitochondrial protein-lipid complexes into organic solvents: inhibition of cytochrome oxidase electron transport by dicyclohexylcarbodiimide and triphenyltin chloride Biochemical and Biophysical Research Communications, 100, 1402-1408 * .

Darszon,A. Vandenberg,C.A. Schonfeld,M. Ellisman,M.H. Spitzer,N.C. Montal,M. 1980. Reassembly of protein-lipid complexes into large bilayer vesicles: perspectives for membrane reconstitution Proceedings of the National Academy of Sciences of the United States of America, 77, 239-243 * .

Darszon,A. 1980. Channel activity in bilayers made from a purified rhodopsin-phosphatidylcholine complex Annals of the New York Academy of Sciences, 358, 337 * .

Ramakrishnan,V. Darszon,A. Philipp,M. Montal,M. 1980. Rhodopsin in model membranes: the kinetics of channel opening and closing in rhodopsin-containing planar lipid bilayers Annals of the New York Academy of Sciences, 358, 36-42 * .

Darszon,A. Vandenberg,C.A. Ellisman,M.H. Montal,M. 1979. Incorporation of membrane proteins into large single bilayer vesicles. Application to rhodopsin Journal of Cell Biology, 81, 446-452 * .

Darszon,A. Blair,L. Montal,M. 1979. Purified rhodopsin-phosphatidylcholine complex in hexane: formation and characterization FEBS Letters, 107, 213-216 * .

Darszon,A. Strasser,R.J. Montal,M. 1979. Rhodopsin--phospholipid complexes in apolar environments: photochemical characterization Biochemistry, 18, 5205-5213 * .

Darszon,A. Philipp,M. Zarco,J. Montal,M. 1978. Rhodopsin-phospholipid complexes in apolar solvents: Formation and properties Journal of Membrane Biology, 43, 71-90 * .

Trissl,H.W. Darszon,A. Montal,M. 1977. Rhodopsin in model membranes: charge displacements in interfacial layers Proceedings of the National Academy of Sciences of the United States of America, 74, 207-210 * .

Darszon,A. Montal,M. Philipp,M. 1977. Formation of detergent-free proteolipids from biological membranes: application to rhodopsin FEBS Letters, 74, 135-138 * .

Montal,M. Darszon,A. Trissl,H.W. 1977. Transmembrane channel formation in rhodopsin-containing bilayer membranes Nature, 267, 221-225 * .

Darszon,A. Montal,M. Zarco,J. 1977. Light increases the ion and non-electrolyte permeability of rhodopsin-phospholipid vesicles Biochemical and Biophysical Research Communications, 76, 820-827 * .

Klip,A. Darszon,A. Montal,M. 1976. Labelling of rhodopsin moieties confined to the membrane lipid bilayer Biochemical and Biophysical Research Communications, 72, 1350-1358 * .

Libros y capítulos recientes

Ramirez-Gomez,H.V. Tuval,I. Guerrero,A. Darszon,A. 2019. Analysis of sperm chemotaxis en: Methods in Cell Biology. Academic Press Inc.. pags. 473-486

Rodriguez-Miranda,E. Acevedo-Fernandez,J.J. Negrete-Leon,E. Darszon,A. Beltran,C. 2019. Efecto de la aloxana en la reaccion acrosomal del espermatozoide de rata en: Investigacion en la Educacion Superior,: Morelia 2019. Memorias del Congreso Internacional de Investigación Academia Journals Morelia 2019, Morelia, Michoacán, México 15 al 17 de mayo de 2019. 214. pags. 2517-2521

Rodriguez-Miranda,E. Acevedo-Fernandez,J.J. Negrete-Leon,E. Darszon,A. Beltran,C. 2018. Expresión diferencial de proteínas en el espermatozoide de ratas diabéticas en: Memorias del Congreso Internacional de Investigación Academia Journals Celaya 2018 vol 28. pags. 4683-4688

Ehrlich,H. Montoya,F. Hernandez,P. Darszon,A. Corkidi,G. 2017. Análisis de Métricas Sobre el Flagelo de Espermatozoides de Humano en 3D para su Clasificación entre Activados e Hiperactivados en: Memorias del XL Congreso Nacional de Ingenieria Biomedica CNIB 2017. pags. 182-185

Hernandez-Herrera,P. Montoya,F. Rendon,J.M. Darszon,A. Corkidi,G. 2017. Sperm Flagellum Center-Line Tracing in Fluorescence 3D+t Low SNR Stacks Using an Iterative Minimal Path Method en: Cheriet,F. Image Analysis and Recognition: 14th International Conference, ICIAR 2017, Montreal, QC, Canada, July 5-7, 2017, Proceedings. Cham. Springer International Publishing. pags. 437-445

Beltran,C. Trevino,C.L. Mata-Martinez,E. Chavez,J.C. Sanchez-Cardenas,C. Baker,M. Darszon,A. 2016. Role of Ion Channels in the Sperm Acrosome Reaction en: Buffone,M.G. Sperm Acrosome Biogenesis and Function During Fertilization. Springer International Publishing Switzerland. pags. 35-69

Wood,C.D. Guerrero,A. Priego-Espinosa,D.A. Martinez-Mekler,G. Carneiro,J. Darszon,A. 2015. Sea Urchin Sperm Chemotaxis en: Cosson,J.J. Flagellar Mechanics and Sperm Guidance. pags. 135-182

Silva-Villalobos,F. Pimentel,J.A. Darszon,A. Corkidi,G. 2014. Imaging of the 3D dynamics of flagellar beating in human sperm en: Engineering in Medicine and Biology Society (EMBC), 2014 36th Annual International Conference of the IEEE. pags. 190-193

Trevino,C.L. Orta,G. Figueiras-Fierro,D. De la Vega-Beltran JL Ferreira,G. Balderas,E. Jose,O. Darszon,A. 2014. Cl- channels and transporters in sperm physiology en: Iwano,M. Sexual Reproduction in Animals and Plants. Tokyo. Springer-Japan. pags. 59-84

Santi,C. Darszon,A. 2012. Slo3 channels unique to spermatozoa and essential en: Morisawa,M. Sperm Cell Research in the 21st Century: Historical Discoveries to New Horizons,11th International Symposium on Spermatology. Tokyo. ISS. pags. 23-28

Corkidi,G. Taboada,B. Wood,C.D. Guerrero,A. Darszon,A. 2008. Three-dimensional image acquisition system for multi-sperm tracking en: Biomedical Imaging: From Nano to Macro, 2008. ISBI 2008. 5th IEEE International Symposium on. IEEE. pags. 145-148

Trevino,C.L. Escobar,L. Vaca,L. Morales-Tlalpan,V. Ocampo,A.Y. Darszon,A. 2008. Maitotoxin: a Unique Pharmacological Tool for Elucidating Ca2+-Dependent Mechanisms, en: Botana,L.M. Seafood and Freshwater Toxins: Pharmacology, Physiology, and Detection. Boca Raton. CRC Press. pags. 503-516

Darszon,A. 2008. Canales, iones y cómo el espermatozoide interpreta los mensajes del óvulo en: *agustin Una ventana al quehacer científico. Instituto de Biotecnología de la UNAM 25 aniversario, cap 3. Mexico, D.F.. UNAM. pags. 29-42

Felix,R. Lopez-Gonzalez,I. Munoz-Garay,C. Darszon,A. 2004. Ion channels and sperm function en: Maue,R.A. Molecular Insights into Ion Channel Biology in Health and Disease. Elsevier. pags. 407-431

Darszon,A. Lopez,I. Trevino,C.L. 2003. Diálogo entre el espermatozoide y el óvulo en: Martínez-Palomo A Fronteras de la Biología en los Inicios del Siglo XXI, Biología Celular, Genética Molecular y Biotecnología, Módulo II.. Mexico,D.F.. El Colegio Nacional. pags. 33-55

Darszon,A. Espinosa,F. Galindo,B. Sanchez,D. Beltran,C. 2002. Regulation of sperm ion currents en: Hardy,D. Fertilization. Nueva York, Londres, Tokio. Academic Press. pags. 225-264

Lievano,A. Darszon,A. 2001. Ion Channels in Sperm en: Sperelakis,N. Cell Physiology Sourcebook: A Molecular Approach. San Diego. Academic Press. pags. 509-522

Labarca,P. Santi,C. Zapata,O. Beltran,C. Lievano,A. Sandoval,Y. Darszon,A. 1997. 9 en: Saez,J.C. From ion channels to cell-to-cell conversations. New York. Plenum Press. pags. 147-168

Darszon,A. Santi,C.M. Serrano,C.J. Trevino,C.L. HernandezCruz,A. Lievano,A. 1997. T-type Ca2+ channels expressed during mouse spermatogenesis may mediate sperm acrosome reaction en: Baker,G.W.H. VIth International Congress of Andrology. Bologna, Italia. Monduzzi Editore. pags. 165-170

Lievano,A. Darszon,A. 1995. Ion Channels in Sperm en: Sperelakis,N. Cell physiology source book. Academic Press. pags. 330-339

Darszon,A. 1986. [36] Planar bilayers: A powerful tool to study membrane proteins involved in ion transport en: Packer,L. Methods in Enzymology. Academic Press. pags. 486-502 *

Gomez-Puyou,A. Beltran,C. Darszon,A. de Gomez-Puyou,M.T. 1984. Modulation of the catalytic properties of the mitochondrial ATpase by its inhibitor protein en: Packer,L. H+-ATPase (ATP synthase)--structure, function, biogenesis : the F0F1 complex of coupling membranes. Bari. Published on behalf of the ICSU Press by Adriatica Editrice. pags. 397-409 *

Montal,M. Darszon,A. Strasser,R. 1978. RHODOPSIN AND BACTERIORHODOPSIN IN MODEL MEMBRANES en: Scarpa,A. Frontiers of Biological Energetics. Academic Press. pags. 1109-1117 *

Otras Publicaciones

Luque,G.M. Romarowski,A. Gervasi,M.G. Orta,G. de la Vega-Beltran,J. Stival,C. Gilio,N. Dalotto-Moreno,T. Krapf,D. Visconti,P.E. Krapf,D. Darszon,A. Buffone,M.G. 2020. Cdc42 activity is essential for the interplay between cAMP/PKA pathway and CatSper function bioRxiv, Preprint posted August 25, 2020, .

Corkidi,G. Hernandez-Herrera,P. Montoya,F. Gadelha,H. Darszon,A. 2020. Computer-assisted segmentation-free analysis for long-term assessment of flagellar beating in free swimming human spermatozoa: the role of emptying calcium stores bioRxiv, Preprint posted July 15, 2020.

Gadelha,H. Hernandez-Herrera,P. Montoya,F. Darszon,A. Corkidi,G. 2019. The human sperm beats anisotropically and asymmetrically in 3D bioRxiv, Preprint posted October 05, 2019, 795245.

Garza-Lopez,E. Aldana,A. Darszon,A. Nishigaki,T. Lopez-Gonzalez,I. 2019. CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in their domains I/II bioRxiv, Preprint posted May 02, 2019, 626069.

Bondarenko,O. Corzo,G. Santana,F.L. del Rio-Portilla,F. Darszon,A. Lopez-Gonzalez,I. 2019. Non-enzymatically oxidized arachidonic acid regulates mouse spermatogenic cell T-type Ca2+ currents bioRxiv, Preprint posted March 23, 2019. [Publicado Febs Letters 2019], 586297.

Corkidi-Blanco,G. Darszon-Israel,A. 2019. El flagelo del espermatozoide humano en tres dimensiones Biotecnología en Movimiento.Revista de divulgación del Instituto de Biotecnología de la UNAM, 16, 3-5.

Priego-Espinosa,D.A. Darszon,A. Guerrero,A. Gonzalez-Cota,A.L. Nishigaki,T. Martinez-Mekler,G. Carneiro,J. 2018. Modular mathematical analysis of the control of flagellar Ca2+-spike trains produced by CatSper and Cav channels in sea urchin sperm bioRxiv, preprint posted October 18, 2018, .

Ramirez-Gomez,H.V. Jimenez-Sabinina,V. Tuval,I. Velazquez-Perez,M. Beltran,C. Carneiro,J. Wood,C. Darszon,A. Guerrero,A. 2017. Sperm chemotaxis is driven by the slope of the chemoattractant concentration field bioRxiv, preprint posted August 11, 2019., .

* Indica publicación con otra institución de adscripción