Group of Dr. Enrique Morett

Curriculum vitae (English)

Instituto de Biotecnología

Universidad Nacional Autónoma de México

Departamento de Reconocimiento Molecular y Bioestructura

Research Interests:

Molecular Genetics (regulation of transcription initiation), Molecular Evolution (general protein evolution and evolution of the catalitic activity), and the like.

Morett´s Group

From top-left to bottom-right Rene Hernandez, Technitian, Sequencing facility; Humberto Flores, Assitant Researcher; Lety Olvera, Technitian; Ricardo Grande, Assitant Researcher; Juana Ferrer, Laboratory Assistant; Francisco Reyes, Laboratory Assistant; Yagul Pedraza, Student; Esmeralda Lopez, Student; Angel Ernesto Dago, Student; Christian Torres, Student; Alfredo Mendoza, Student; Emmanuel Rajan Koil, Posdoc; Enrique Morett, myself; Nelly Mellado, Accounts Assistant and Mary Olvera, Technitian.

Recent papers of the group:

1. Ciria R, Abreu-Goodger C, Morett E, Merino E. (2004). GeConT: gene context analysis. Bioinformatics. Apr 8 [Epub ahead of print].

GeConT Gene Context Tool

2. Morett E, Korbel JO, Rajan E, Saab-Rincon G, Olvera L, Olvera M, Schmidt S, Snel B, Bork P. (2003). Systematic discovery of analogous enzymes in thiamin biosynthesis. Nature Biotechnol. 21:790-795.

This paper has been commented in Faculty of 1000

3. Chaney M, Grande R, Wigneshweraraj SR, Cannon W, Casaz P, Gallegos MT, Schumacher J, Jones S, Elderkin S, Dago AE, Morett E y Buck M. (2001). Binding of Transcription Activators to the Enhancer Dependent Sigma 54 Protein in the Presence of ADP-Aluminum Fluorate. Genes and Development 15:2282-2294.

4. Juarez K. Flores H, Davila S, Olvera L, Gonzalez V. and Morett E. (2000). Reciprocal Domain Evolution of a Transactivator Protein in a Restricted Sequence Landscape. Proc Natl Acad Sci USA.97:3314-3318.

This paper has been commented in Mexico City's newspaper La Jornada "Cuatro mil millones de años en busca de lo óptimo"

5. Grande R, Valderrama B. and Morett E. (1999). Suppression Analysis of Positive Control Mutants of NifA Revealed two Overlapping Promoters for rpoN in Klebsiella pneumoniae. J. Mol. Biol.294:291-298.

6. Barrios H, Valderrama B. and Morett E. (1999). Compilation and Analysis of sigma-54 Dependent Promoter Sequences. Nucleic Acids Res.27:4305-4313.

7.Morett E. and Bork, P. (1999). A Novel Transactivation Domain in Parkin. TIBS. 24:229-231.

8.Morett E. and Bork, P. (1998). Evolution of New Protein Function: Recombinational Enhancer Fis Originated by Horizontal Gene Transfer from the Transcriptional Regulator NtrC. FEBS Lett. 433:108-112.

9. Gonzalez V, Olvera L, Soberon X and Morett E (1998). In vivo Studies on the Positive Control of NifA: An Hydrophobic Aminoacid Patch at the Central Domain Involved in Transcriptional Activation. Mol Microbiol. 28:55-68.

10. Barrios H, Grande R, Olvera, L and Morett E. (1998). In vivo Genomic footprinting and Chemical Proobing Reveal that the Bradyrhizobium japonicum fixRnifA Promoter Region is Differentially Occupied and Melted by two Distinct RNA Polymerase Holoenzymes. Proc Natl Acad Sci USA. 95:1014-1019.

11. Osuna J, Soberón X and Morett E. (1997). A Proposed Architecture for the Central Domain of the Bacterial Enhancer-Binding Proteins based on Secondary Structure Prediction and Fold Recognition. Prot. Sci. 6:543-555.

12. del Rio G, Morett E and Soberon X. (1997). Did Cyclodextrin Glycosyltransferases Evolved from alpha-Amylases? FEBS Lett. 416:221-224.

The lab

We share this lab with Dr, Xavier Soberon, Director of the Institute. Our groups have common interest in molecular evolution of proteins and catalysis.
The laboratory has 16 individual working benches, each one with a desk, and several meters of lateral benches. Aditionally, the lab has five offices, three rooms for comun equipment, a lab dedicated to work with radioisotops, and a large bench with computers. The Institute has several floor centrifuges, ultracentrifuges, cold rooms, temperature-controlled rooms, a DNA synthesis unit, a polypeptide synthesis unit, an animal house, electron and confocal microscopy facilities, dark room, central computing unit for institutional service, etc.
The major equipment includes digital gel recording set, spectrograph, spin vacum dryers, fume cave, steril benches, several thermocyclers, ultrafreecers, autoclave, HPLC, automatic DNA sequencing aparatus, several power packs, electroporators, incubators temperature-controlled baths, analytical balances, sonicator, refrigerated bench centrifugues, microwave ovens,several electrophoresis chambers, etc. We have several Macs, PC's and Silicon Graphyc computers.
Reactives room
Reactives room
FPLC, AKTA (Pharmacia) for protein purification.
Automatic DNA Sequencing unit.

Group Members

Lety. She controls everybody and everything in the lab.
Mary, she is in charge of preparing samples for automatic sequencing and collaborates in directed evolution projects.
Here she is reading about reptiles, her most profound interest in life.
Ricardo finished his Ph D last July and continued in the group now as research staff.
Ricardo. It´s hard to see when he is working.
Isa, She was visiting the group. She comes from Alicante University, Spain. She plans to come again soon! (esa cara de espanto no es la mas usual!!)
Isa, from Choni´s Lab.
He is Angel Ernesto. He is from Cuba and he is doing his Ph D here.
Rene Hernandez. This guy is in control of our DNA Sequencer.
Rene Hernandez.
Some useful academic links:

And now, other interesting links. Suggestions welcome

This little guy is Andres (mi hijo) esta guapo, verdad?
A more recent picture of Andres
He´s Diego, my elder son.
Some art by Andres
Noticias de México y del Mundo
Universidad de Guadalajara.
This is the best server in Gabriel Moreno's opinion (he comes from Guadalajara!).
Want to know about Mexico? This is where (Culture, cuisine, painters, and a big ETCETERA).
Access to the WebMuseum
Sunsite. Use this or the one above to find your nearest mirror.
World-Wide Web Home